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Rabbit抗原名称
HP1α分子别名
Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha (HP1 alpha); CBX5; HP1A免疫原
Synthetic Peptide细胞定位
NucleusAccession
P45973克隆号
S-1462-53抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, 293T, MCF7, NIH/3T3, C6纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
ChIP
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Rt ICC 1:500 Hu, Ms ChIP 1:20-1:50
HP1α (heterochromatin protein 1 alpha) is a structural chromosomal protein that plays a multifaceted role in chromatin regulation and gene expression. It is primarily associated with heterochromatin, a condensed form of chromatin that promotes gene silencing, particularly at telomeres and centromeres. HP1α binds to the histone modification H3K9me3 (histone H3 lysine 9 trimethylation) via its N-terminal chromodomain, which is crucial for the assembly and maintenance of heterochromatin. This binding helps to compact chromatin, control gene expression, and maintain genome integrity. In addition to its role in gene silencing, HP1α also has the capacity to promote gene expression. It can impact the expression of genes in euchromatin as well as heterochromatic genes. The mechanisms by which HP1α can positively regulate gene expression include maintenance of heterochromatin structure and facilitating transcriptional elongation. HP1α is also involved in the induction of heat-shock genes and has been shown to interact directly with RNA polymerase II and heterogeneous nuclear ribonuclear proteins (hnRNPs), suggesting a role in RNA processing.
免疫印迹
WB result of HP1α Recombinant Rabbit mAb
Primary antibody: HP1α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 22 kDa
Observed MW: 22 kDaWB result of HP1α Recombinant Rabbit mAb
Primary antibody: HP1α Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 22 kDa
Observed MW: 22 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling HP1 alpha antibody at 1/500 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- HP1 alpha antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- HP1 alpha antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1%
formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used HP1 alpha Recombinant Rabbit mAb (S-1462-53)and IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA/input DNA) showed the enrichment of WDR6, TAF6 and SAT-α in
HP1 alpha Recombinant Rabbit mAb (S-1462-53)-immunoprecipitated sample.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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