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Rabbit抗原名称
CD3L1 (ITPRIPL1)分子别名
Inositol 1,4,5-trisphosphate receptor-interacting protein-like 1; ITPRIPL1; KIAA1754L免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q6GPH6克隆号
S-1716-50抗体类型
Recombinant mAb抗体同种型
IgG应用
WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu 反应种属 ?
Hu阳性样本
Raji, HCT 116, A375纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
CD3L1 (CD3 ligand 1), also known as ITPRIPL1, is the first identified ligand of CD3 and plays a crucial role in tumor immune evasion and testicular immune privilege. CD3L1 inhibits T-cell activation by directly binding to CD3ε, a process that involves the sustained binding of Nck to the intracellular domain of CD3ε, competitively inhibiting the recruitment and phosphorylation of Zap70, thus suppressing T-cell activation at the initial stage. This discovery reveals the existence of a natural ligand for CD3, suggesting that the TCR/CD3 complex may not be regulated in a TCR-dominant "monopolar" manner but rather in a "bipolar" mode where both TCR and CD3 have natural regulatory ligands. The identification of CD3L1 offers a new target for cancer immunotherapy and promises to bring new breakthroughs in the field.
免疫印迹
WB result of CD3L1 (ITPRIPL1) Recombinant Rabbit mAb
Primary antibody: CD3L1 (ITPRIPL1) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: A375 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 55 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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