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ATPB Rabbit Polyclonal Antibody

ATP synthase subunit beta,mitochondrial,ATP synthase F1 subunit beta,ATP5F1B,ATP5B,ATPMB,ATPSB

货号： S0B1226

Datasheet COA
  • 价格： ￥400
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    ATPB

  • 分子别名

    ATP synthase subunit beta, mitochondrial; ATP synthase F1 subunit beta; ATP5F1B; ATP5B; ATPMB; ATPSB

  • 免疫原

    Synthetic Peptide

  • Accession

    P06576

  • 抗体类型

    Polyclonal antibody

  • 抗体同种型

    IgG

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, HepG2, 293T, HT-29, HL-60, LoVo, mouse liver, rat liver, rat heart

  • 预测反应种属
    (反应种属缩写表)

    Almost all species

  • 纯化方式

    Immunogen Affinity

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:5000 Hu, Ms, Rt
IHC-P 1:250 Hu, Ms, Rt
背景介绍

  • ATPB refers to the "ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide," which is a subunit of the mitochondrial ATP synthase enzyme. This enzyme is crucial for cellular energy production as it catalyzes the synthesis of ATP, utilizing the electrochemical gradient of protons across the inner mitochondrial membrane during oxidative phosphorylation. ATPB is one of the subunits that make up the F1 complex, the soluble catalytic core of ATP synthase, which is composed of five different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other three. The protein is also known by several synonyms including ATP5B, ATPMB, ATPSB, and is associated with the gene symbol ATPB. The molecular weight of the protein is approximately 57 kDa, with a calculated molecular weight of 529 amino acids. ATPB plays a significant role in the process of ATP synthesis within the mitochondria, highlighting its importance in cellular metabolism and energy production.

  • 免疫印迹

    • WB result of ATPB Rabbit pAb
      Primary antibody: ATPB Rabbit pAb at 1/5000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HepG2 whole cell lysate 20 µg
      Lane 3: 293T whole cell lysate 20 µg
      Lane 4: HT-29 whole cell lysate 20 µg
      Lane 5: HL-60 whole cell lysate 20 µg
      Lane 6: LoVo whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 56 kDa
      Observed MW: 50 kDa

    • WB result of ATPB Rabbit pAb
      Primary antibody: ATPB Rabbit pAb at 1/5000 dilution
      Lane 1: mouse liver lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 56 kDa
      Observed MW: 50 kDa

    • WB result of ATPB Rabbit pAb
      Primary antibody: ATPB Rabbit pAb at 1/5000 dilution
      Lane 1: rat liver lysate 20 µg
      Lane 2: rat heart lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 56 kDa
      Observed MW: 50 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung carcinoma. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat stomach. Anti-ATPB antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of ATPB in tumor tissue.

    • Expression of ATPB in human tissue.

    • Expression of ATPB in mouse & rat tissue.

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