HexokinaseⅠRecombinant Rabbit mAb (S-868-113)

Brain form hexokinase,Hexokinase type I (HK I),Hexokinase-A,HK1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1225

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

HexokinaseⅠ
分子别名

Brain form hexokinase; Hexokinase type I (HK I); Hexokinase-A; HK1
免疫原

Synthetic Peptide
细胞定位

Mitochondrion outer membrane
Accession

P19367
克隆号

S-868-113
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms
阳性样本

293T, HeLa, Jurkat, MCF7, U-87 MG, NIH/3T3, C2C12
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms
ICC 1:500 Hu
ICFCM 1:50

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms
ICC	1:500	Hu
ICFCM	1:50

背景介绍

Hexokinase I (HK-I) is a subtype of the hexokinase family. The primary function of HK-I is to regulate the entry of glucose into the glycolytic pathway and generate ATP. It is firmly bound to mitochondria and, in the brain, HK-I catalyzes the phosphorylation of glucose, determining the rate of glycolysis in the brain. HK-I also acts as a coordinating enzyme to prevent an imbalance between glycolysis and mitochondrial oxidative reactions, which could lead to the accumulation of lactic acid. Additionally, in hematopoietic cell lines, HK-I is co-expressed with GLUT1, increasing the level of glucose phosphorylation and the level of cellular nicotinamide adenine dinucleotide phosphate (NADPH), thereby exerting an anti-apoptotic effect.

免疫印迹
- WB result of HexokinaseⅠRecombinant Rabbit mAb
  Primary antibody: HexokinaseⅠRecombinant Rabbit mAb at 1/1000 dilution
  Lane 1: 293T whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Lane 4: MCF7 whole cell lysate 20 µg
  Lane 5: U-87 MG whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 102 kDa
  Observed MW: 110 kDa
- WB result of HexokinaseⅠRecombinant Rabbit mAb
  Primary antibody: HexokinaseⅠRecombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: C2C12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 102 kDa
  Observed MW: 110 kDa
流式分析
- Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Hexokinase 1 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- Hexokinase 1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).