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Rabbit抗原名称
CRALBP分子别名
Retinaldehyde-binding protein 1; Cellular retinaldehyde-binding protein; RLBP1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P12271抗体类型
Polyclonal antibody抗体同种型
IgG应用
IHC-P, ICC, WB, IF反应种属 ?
Hu, Ms, Rt阳性样本
SK-MEL-2, SK-MEL-28, mouse eye, rat eye预测反应种属
(反应种属缩写表)Bv纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Ms, Rt |
| ICC | 1:500 | Hu, Ms, Rt |
| IF | 1:1000 | Ms, Rt |
CRALBP, which stands for Cellular Retinaldehyde-Binding Protein, is a protein that plays a critical role in the visual cycle, particularly in the regeneration of 11-cis-retinal for use in rod visual pigments such as opsin and rhodopsin. It is expressed in the retinal pigment epithelium (RPE) and Müller glia (MG) and is essential for maintaining visual function. CRALBP binds to 11-cis-retinol in the visual cycle, thereby augmenting the activity of retinoid isomerohydrolase RPE65 and facilitating the oxidation of 11-cis-retinol to 11-cis-retinal. This process is vital for the photoisomerization of 11-cis-retinal to all-trans-retinal, which is a key step in the visual cycle. In terms of therapeutic implications, gene therapy targeting the RLBP1 gene, which encodes CRALBP, has shown promise in preclinical studies. For instance, gene therapy with adeno-associated viral (AAV) vectors expressing a corrected RLBP1 gene (AAV8-RLBP1) has been shown to restore CRALBP levels and improve dark adaptation in Rlbp1-deficient mice. This suggests potential for treating retinal dystrophies associated with CRALBP dysfunction.
免疫印迹
WB result of CRALBP Rabbit pAb
Primary antibody: CRALBP Rabbit pAb at 1/1000 dilution
Lane 1: SK-MEL-2 whole cell lysate 20 µg
Lane 2: SK-MEL-28 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 36 kDa
Observed MW: 36 kDaWB result of CRALBP Rabbit pAb
Primary antibody: CRALBP Rabbit pAb at 1/1000 dilution
Lane 1: mouse eye lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 36 kDa
Observed MW: 36 kDaWB result of CRALBP Rabbit pAb
Primary antibody: CRALBP Rabbit pAb at 1/1000 dilution
Lane 1: rat eye lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 36 kDa
Observed MW: 36 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse retina. Anti-CRALBP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat retina. Anti-CRALBP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in SK-MEL-28 cells. Anti- CRALBP antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded mouse retina. Anti-CRALBP antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat retina. Anti-CRALBP antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
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