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宿主来源
Rabbit抗原名称
Actin免疫原
Synthetic PeptideAccession
P68133, P60709, P63261, P68032, P62736, P63267克隆号
S-1365-34抗体类型
Recombinant mAb抗体同种型
IgG应用
ICFCM, ICC, WB反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HepG2, A431, MCF7, NIH/3T3, RAW264.7,C6, PC-12纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
| ICFCM | 1:500 | Hu |
Actin protein is a highly abundant protein in eukaryotic cells and plays a critical role in various cellular functions. Actin exists in two forms, Globular Actin (G-actin) and Filamentous Actin (F-actin). G-actin is the monomeric, soluble form of the protein, while F-actin refers to the polymerized, filamentous structure. Actin filaments form a dynamic cytoskeleton that provides structural support, enables cell motility, and supports muscle contraction. In muscles, actin molecules twist together to form thin filaments, which interdigitate with thick filaments composed of myosin, the most abundant protein in muscle. When a signal for muscle contraction is sent along a nerve to a muscle cell, actin and myosin are activated. Myosin acts as a motor, hydrolyzing adenosine triphosphate (ATP) to release energy, which causes a myosin filament to move along an actin filament, resulting in the sliding of the two filaments past each other. Actin filaments are highly concentrated at the cell periphery, forming a three-dimensional network beneath the plasma membrane. This network, composed of actin filaments and associated actin-binding proteins (known as the cell cortex), determines cell shape and is involved in various cell surface activities, including movement.
免疫印迹
WB result of Pan-Actin Recombinant Rabbit mAb
Primary antibody: Pan-Actin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42 kDa
Observed MW: 42 kDaWB result of Pan-Actin Recombinant Rabbit mAb
Primary antibody: Pan-Actin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42 kDa
Observed MW: 42 kDaWB result of Pan-Actin Recombinant Rabbit mAb
Primary antibody: Pan-Actin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42 kDa
Observed MW: 42 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling Actin antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Pan-Actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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