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Trim30a Recombinant Rabbit mAb (S-1753-35)

Tripartite motif-containing protein 30A,Down regulatory protein of interleukin-2 receptor,Tripartite motif-containing protein 30,Rpt-1,Rpt1,Trim30

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1216

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Trim30a
分子别名

Tripartite motif-containing protein 30A; Down regulatory protein of interleukin-2 receptor; Tripartite motif-containing protein 30; Rpt-1; Rpt1; Trim30
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

P15533
克隆号

S-1753-35
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms, Rt
阳性样本

A20, CTLL-2, mouse muscle, mouse heart, mouse thymus
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP

IF ?
稀释度

应用稀释度推荐种属

WB 1:1000 Ms
IP 1:50 Ms
IHC-P 1:200 Ms, Rt
IF 1:500 Ms

应用	稀释度	推荐种属
WB	1:1000	Ms
IP	1:50	Ms
IHC-P	1:200	Ms, Rt
IF	1:500	Ms

背景介绍

TRIM30a, also known as the tripartite motif-containing protein 30 alpha, is a protein that plays a significant role in immune responses and cellular regulation. It has been found to make tumor cells more sensitive to natural killer (NK) cell-mediated immune surveillance. Overexpression of TRIM30a in tumor cells can slow down their growth in immunocompetent mice, but not in mice with depleted NK cells, indicating its role in enhancing the susceptibility of tumor cells to NK cell cytotoxicity. TRIM30a overexpression can impair the protein quality control (PQC) system in tumor cells by hindering the clearance of misfolded proteins and increasing the production of reactive oxygen species, suggesting that TRIM30a could be a potential tumor suppressor and immune modulator. TRIM30a acts as a negative-feedback regulator of the intracellular DNA and DNA virus-triggered response by targeting the stimulator of interferon genes (STING). It promotes the degradation of STING via K48-linked ubiquitination at Lys275, which is crucial for maintaining homeostasis and limiting the risk of autoimmunity.

免疫印迹
- WB result of Trim30a Recombinant Rabbit mAb
  Primary antibody: Trim30a Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: unboiled A20 whole cell lysate 20 µg
  Lane 2: unboiled CTLL-2 whole cell lysate 20 µg
  Lane 3: unboiled mouse muscle lysate 20 µg
  Lane 4: unboiled mouse heart lysate 20 µg
  Lane 5: unboiled mouse thymus lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 57 kDa
  Observed MW: 54 kDa
免疫沉淀
- Trim30a Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Trim30a in 0.4 mg mouse heart lysate.
  Western blot was performed on the immunoprecipitate using Trim30a Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: mouse heart lysate 20 µg (Input)
  Lane 2: Trim30a Rabbit mAb IP in mouse heart lysate
  Lane 3: Rabbit monoclonal IgG IP in mouse heart lysate
  Predicted MW: 57 kDa
  Observed MW: 54 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-Trim30a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Trim30a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-Trim30a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-Trim30a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat spleen. Anti-Trim30a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-Trim30a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse ovary. Anti-Trim30a antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
- Expression of Trim30a in mouse & rat tissue.