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Rabbit抗原名称
Prosurfactant Protein C分子别名
Surfactant protein C; SP-C; Pulmonary surfactant-associated protein C; Pulmonary surfactant-associated proteolipid SPL(Val); SP5; SFTPC; SFTP2免疫原
Synthetic Peptide细胞定位
SecretedAccession
P11686克隆号
S-1773-6抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt阳性样本
mouse lung, rat lung预测反应种属
(反应种属缩写表)Mq纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Prosurfactant Protein C (proSP-C) is a precursor protein that plays a crucial role in the formation of pulmonary surfactant, which is essential for reducing surface tension at the alveolar air-liquid interface and maintaining lung stability during ventilation. ProSP-C is synthesized as a 21 kDa integral membrane precursor protein in alveolar type II cells and is subsequently proteolytically processed to a 3.7 kDa secretory protein, known as Surfactant Protein C (SP-C). SP-C enhances the adsorption and spreading of phospholipids at the air-liquid interface, thereby promoting the surface tension-lowering properties of surfactant.
免疫印迹
WB result of Prosurfactant Protein C Recombinant Rabbit mAb
Primary antibody: Prosurfactant Protein C Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse lung lysate 20 µg
Negative control: A549 whole cell lysate; mouse liver lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 21 kDa
Observed MW: 22 kDaWB result of Prosurfactant Protein C Recombinant Rabbit mAb
Primary antibody: Prosurfactant Protein C Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat lung lysate 20 µg
Negative control: rat liver lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 21 kDa
Observed MW: 21 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human lung. Anti-Prosurfactant Protein C antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Prosurfactant Protein C antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-Prosurfactant Protein C antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Prosurfactant Protein C in tumor tissue.
Expression of Prosurfactant Protein C in human tissue.
Expression of Prosurfactant Protein C in mouse & rat tissue.
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