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Prosurfactant Protein C Recombinant Rabbit mAb (S-1773-6)

Surfactant protein C,SP-C,Pulmonary surfactant-associated protein C,Pulmonary surfactant-associated proteolipid SPL(Val),SP5,SFTPC,SFTP2

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1215
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产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Prosurfactant Protein C

  • 分子别名

    Surfactant protein C; SP-C; Pulmonary surfactant-associated protein C; Pulmonary surfactant-associated proteolipid SPL(Val); SP5; SFTPC; SFTP2

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Secreted

  • Accession

    P11686

  • 克隆号

    S-1773-6

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    mouse lung, rat lung

  • 预测反应种属
    (反应种属缩写表)

    Mq

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    WB

    IP

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Ms, Rt
    IP 1:50 Ms
    IHC-P 1:1000 Hu, Ms, Rt
背景介绍

  • Prosurfactant Protein C (proSP-C) is a precursor protein that plays a crucial role in the formation of pulmonary surfactant, which is essential for reducing surface tension at the alveolar air-liquid interface and maintaining lung stability during ventilation. ProSP-C is synthesized as a 21 kDa integral membrane precursor protein in alveolar type II cells and is subsequently proteolytically processed to a 3.7 kDa secretory protein, known as Surfactant Protein C (SP-C). SP-C enhances the adsorption and spreading of phospholipids at the air-liquid interface, thereby promoting the surface tension-lowering properties of surfactant.

  • 免疫印迹

    • WB result of Prosurfactant Protein C Recombinant Rabbit mAb
      Primary antibody: Prosurfactant Protein C Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: A549 whole cell lysate 20 µg
      Lane 2: mouse liver lysate 20 µg
      Lane 3: mouse lung lysate 20 µg
      Negative control: A549 whole cell lysate; mouse liver lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 21 kDa
      Observed MW: 22 kDa

    • WB result of Prosurfactant Protein C Recombinant Rabbit mAb
      Primary antibody: Prosurfactant Protein C Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: rat liver lysate 20 µg
      Lane 2: rat lung lysate 20 µg
      Negative control: rat liver lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 21 kDa
      Observed MW: 21 kDa

  • 免疫沉淀

    • Prosurfactant Protein C Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Prosurfactant Protein C in 0.4 mg mouse lung lysate.
      Western blot was performed on the immunoprecipitate using Prosurfactant Protein C Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: mouse lung lysate 20 µg (Input)
      Lane 2: Prosurfactant Protein C Rabbit mAb IP in mouse lung lysate
      Lane 3: Rabbit monoclonal IgG IP in mouse lung lysate
      Predicted MW: 21 kDa
      Observed MW: 22 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human lung. Anti-Prosurfactant Protein C antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse lung. Anti-Prosurfactant Protein C antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat lung. Anti-Prosurfactant Protein C antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of Prosurfactant Protein C in tumor tissue.

    • Expression of Prosurfactant Protein C in human tissue.

    • Expression of Prosurfactant Protein C in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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