热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 其他一抗

所有图片（1/3）

BSCL2/Seipin Recombinant Rabbit mAb (S-1669-46)

Bernardinelli-Seip congenital lipodystrophy type 2 protein

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1213
规格
数量
收藏 分享
产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    BSCL2/Seipin

  • 分子别名

    Bernardinelli-Seip congenital lipodystrophy type 2 protein

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Endoplasmic reticulum membrane

  • Accession

    Q96G97

  • 克隆号

    S-1669-46

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms

  • 阳性样本

    293T, HeLa, mouse brain, mouse testis

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution,-20 °C as supplied

  • 应用

    WB

    IP

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    IP 1:50:00
背景介绍

  • Seipin protein is a transmembrane protein that plays a crucial role in the biogenesis of lipid droplets (LDs). It directly affects cellular lipid homeostasis by regulating the mechanisms of fatty acid synthesis, phosphatidic acid (PA) metabolism, or sphingolipid production. Seipin mediates the synthesis of phosphatidylcholine (PC) and regulates lipolysis. Seipin has an evolutionarily conserved role in the biogenesis and degradation of lipid droplets across different species. The absence of Seipin can lead to severe diseases, including the genetic disorder Berardinelli-Seip congenital lipodystrophy type 2 (CGL2).

  • 免疫印迹

    • WB result of BSCL2/Seipin Recombinant Rabbit mAb
      Primary antibody: BSCL2/Seipin Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: 293T whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 44 kDa
      Observed MW: 48 kDa

    • WB result of BSCL2/Seipin Recombinant Rabbit mAb
      Primary antibody: BSCL2/Seipin Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse brain lysate 20 µg
      Lane 2: mouse testis lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 44 kDa
      Observed MW: 48 kDa

  • 免疫沉淀

    • BSCL2/Seipin Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating BSCL2/Seipin in 0.4 mg HeLa whole cell lysate.
      Western blot was performed on the immunoprecipitate using BSCL2/Seipin Recombinant Rabbit mAb at 1/1000 dilution.
      Secondary antibody (HRP) for IP was used at 1/1000 dilution.
      Lane 1: HeLa whole cell lysate 20 µg (Input)
      Lane 2: BSCL2/Seipin Recombinant Rabbit mAb IP in HeLa whole cell lysate
      Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
      Predicted MW: 44 kDa
      Observed MW: 48 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

评论(0)