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Rabbit抗原名称
Phospho-NF-κB p65 (S529)分子别名
Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; RELA; NFKB3免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
Q04206克隆号
S-1125-59抗体类型
Recombinant mAb抗体同种型
IgG应用
WB反应种属 ?
Hu阳性样本
HeLa纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
Phosphorylation of NF-κB p65 at Ser529 is a post-translational modification that plays a significant role in the regulation of NF-κB activity. This modification is catalyzed by casein kinase II (CK2) upon stimulation by cytokines such as interleukin-1β (IL-1β) or tumor necrosis factor α (TNFα), leading to increased p65 transactivation potential in a gene-specific manner. Phosphorylation at S529 enhances the transcriptional activity of NF-κB, which is crucial for regulating various cellular processes including inflammation, immune responses, and cell survival. Dysregulation of NF-κB signaling, including phosphorylation at S529, has been implicated in various diseases, particularly in the context of chronic inflammation and aging. Phosphorylation of p65 at S529 has also been reported to promote tumorigenesis in various tissues, including the bone, breast, and head and neck, suggesting a significant role of NF-κB phosphorylation in different types of cancers.
免疫印迹
WB result of Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 20 ng/ml TNF-alpha for 5 miniutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 60 kDa
Observed MW: 65 kDa
斑点杂交
Dot blot result of Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb
Lane 1: NF-κB p65 (S529) phospho peptide
Lane 2: NF-κB p65 unmodified peptide
Primary antibody: Phospho-NF-κB p65 (S529) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
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