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KDM6A Recombinant Rabbit mAb (S-1728-51)

Lysine-specific demethylase 6A,Histone demethylase UTX,Ubiquitously-transcribed TPR protein on the X chromosome,Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein,[histone H3]-trimethyl-L-lysine(27) demethylase 6A,UTX

货号： S0B1209

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    KDM6A

  • 分子别名

    Lysine-specific demethylase 6A; Histone demethylase UTX; Ubiquitously-transcribed TPR protein on the X chromosome; Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein; [histone H3]-trimethyl-L-lysine(27) demethylase 6A; UTX

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    O15550

  • 克隆号

    S-1728-51

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    PANC-1, HEK-293, K562, mouse spleen

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms
IHC-P 1:200 Hu, Ms, Rt
背景介绍

  • KDM6A is a histone demethylase that belongs to the KDM6 family, specifically targeting di- and tri-methylated histone H3 lysine 27 (H3K27) to remove these modifications, thereby affecting gene expression and transcriptional regulation. KDM6A is frequently mutated in various cancers and functions as a tumor suppressor. Its loss of function or mutations have been implicated in the development and progression of cancers such as multiple myeloma, esophageal squamous cell carcinoma, clear cell renal cell carcinoma, medulloblastoma, adenoid cystic carcinoma, urothelial bladder cancer, T-cell acute lymphoblastic leukemia, and pancreatic cancer. In prostate cancer, KDM6A mutations are associated with the progression to lethal castration-resistant disease. KDM6A is important for the differentiation of embryonic stem cells and the development of various tissues. Its mutations are known to cause Kabuki syndrome, which is characterized by distinct facial features, skeletal anomalies, and intellectual disability. Apart from its demethylase activity, KDM6A also modulates chromatin reprogramming and mediates transcriptional activation/repression in a ligand protein-dependent manner, affecting various physiological and pathological processes.

  • 免疫印迹

    • WB result of KDM6A Recombinant Rabbit mAb
      Primary antibody: KDM6A Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: THP-1 whole cell lysate 20 µg
      Lane 2: PANC-1 whole cell lysate 20 µg
      Lane 3: HEK-293 whole cell lysate 20 µg
      Lane 4: K562 whole cell lysate 20 µg
      Negative control: THP-1 whole cell lysate
      Weak expression: PANC-1 whole cell lysate
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 154 kDa
      Observed MW: 180 kDa

    • WB result of KDM6A Recombinant Rabbit mAb
      Primary antibody: KDM6A Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: mouse spleen lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 154 kDa
      Observed MW: 180 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-KDM6A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse testis. Anti-KDM6A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat testis. Anti-KDM6A antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of KDM6A in tumor tissue.

    • Expression of KDM6A in human tissue.

    • Expression of KDM6A in mouse & rat tissue.

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