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宿主来源
Rabbit抗原名称
GLS2分子别名
Glutaminase liver isoform, mitochondrial; GLS; L-glutaminase; L-glutamine amidohydrolase; GA免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
Q9UI32抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
Mouse liver, rat liver纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt
GLS2 is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. It plays an important role in the regulation of glutamine catabolism. It promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. It also increases cellular anti-oxidant function via NADH and glutathione production. GLS2 has been implicated in various cancers. It is overexpressed in triple-negative basal-like breast cancer (TNBC) and metastatic lung cancer, and it confers radioresistance in advanced human cervical cancer cells. GLS2 may also reduce reactive oxygen species (ROS) levels by increasing the level of cellular reduced glutathione (GSH), NADH, or NADPH, suggesting a role in cancer metabolism.
免疫印迹
WB result of GLS2 Rabbit pAb
Primary antibody: GLS2 Rabbit pAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Negative control: mouse spleen lysate; mouse kidney lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 66 kDa
Observed MW: 60 kDaWB result of GLS2 Rabbit pAb
Primary antibody: GLS2 Rabbit pAb at 1/1000 dilution
Lane 1: rabbit spleen lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Lane 3: rat liver lysate 20 µg
Negative control: rabbit spleen lysate; rat kidney lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 66 kDa
Observed MW: 60 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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