ENT2 Rabbit Polyclonal Antibody
Equilibrative nucleoside transporter 2,hENT2,36 kDa nucleolar protein HNP36,Delayed-early response protein 12,Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter (Equilibrative NBMPR-insensitive nucleoside transporter),Hydrophobic nucleolar protein,36 kDa,Nucleoside transporter,ei-type,Solute carrier family 29 member 2,SLC29A2,DER12,HNP36
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宿主来源
Rabbit抗原名称
ENT2分子别名
Equilibrative nucleoside transporter 2; hENT2; 36 kDa nucleolar protein HNP36; Delayed-early response protein 12; Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter (Equilibrative NBMPR-insensitive nucleoside transporter); Hydrophobic nucleolar protein, 36 kDa; Nucleoside transporter, ei-type; Solute carrier family 29 member 2; SLC29A2; DER12; HNP36免疫原
Synthetic Peptide细胞定位
Apical cell membraneAccession
Q14542抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Rt阳性样本
HepG2, K562, Jurkat, SH-SY5Y纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:10000 Hu IP 1:50 Hu IHC-P 1:250 Hu, Ms, Rt ICC 1:500 Hu ICFCM 1:500 Hu
ENT2, or Equilibrative Nucleoside Transporter 2, is a bidirectional transporter embedded in the biological membrane and is found in most tissue and cell types. It plays a crucial role in mediating the uptake of purine and pyrimidine nucleosides and nucleobases, as well as transporting a variety of nucleoside-derived drugs, which are predominantly used in anticancer therapy. The high expression of ENT2 has been correlated with advanced stages of different types of cancers, making it a significant interest as a potential therapeutic target. Furthermore, ENT2 is known to play critical roles in signaling pathways and cell cycle progression.
免疫印迹
WB result of ENT2 Rabbit pAb
Primary antibody: ENT2 Rabbit pAb at 1/10000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 50 kDa
Observed MW: 60~70 kDa
流式分析
Flow cytometric analysis of K562 (Human chronic myelogenous leukemia lymphoblast) labelling ENT2 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
ENT2 Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ENT2 in 0.4 mg K562 whole cell lysate.
Western blot was performed on the immunoprecipitate using ENT2 Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: K562 whole cell lysate 20 µg (Input)
Lane 2: ENT2 Recombinant Rabbit mAb IP in K562 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in K562 whole cell lysate
Predicted MW: 50 kDa
Observed MW: 60~70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-ENT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-ENT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-ENT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-ENT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-ENT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-ENT2 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in K562 cells. Anti-ENT2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of ENT2 in human tissue.
Expression of ENT2 in tumor tissue.
Expression of ENT2 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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