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Rabbit抗原名称
Cyclin B1分子别名
G2/mitotic-specific cyclin-B1; CCNB1; CCNB免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P14635克隆号
S-1407-224抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, HEK-293, U-2 OS纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu ICC 1:500 Hu ICFCM 1:50 Hu
Cyclin B1 is a crucial regulatory protein involved in the cell cycle, particularly in controlling the G2/M transition phase. It forms transiently active kinase complexes with CDK1 (Cyclin-dependent kinase 1) that play distinct roles in mitosis. It accumulates during S-G2, shuttles between the cytoplasm and nucleus, and becomes concentrated in the nucleus in late G2. During prometaphase, Cyclin B1 localizes to centrosomes, mitotic spindle, and chromosomes. Cyclin B1 is involved in the attachment of kinetochore fibers (k-fibers) formed by microtubules during mitosis. It is degraded after the completion of microtubule attachment to kinetochores during mitosis and inactivation of the spindle assembly checkpoint. Uncontrolled expression of Cyclin B1 is associated with neoplastic transformation and the development of gynecological cancers. It has been implicated in the progression of various types of cancer, including gastric, breast, and non-small cell lung cancer. Elevated Cyclin B1 expression correlates with decreased overall survival in multiple cancer types. Cyclin B1/CDK1 is also involved in mitochondrial bioenergetics, which plays a pivotal role in tumor radiation response and resistance to anti-cancer therapies.
免疫印迹
WB result of Cyclin B1 Recombinant Rabbit mAb
Primary antibody: Cyclin B1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: U-2 OS whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 48 kDa
Observed MW: 55 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling Cyclin B1 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human placenta. Anti-Cyclin B1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Cyclin B1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti-Cyclin B1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Cyclin B1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Cyclin B1 in human tissue.
Expression of Cyclin B1 in tumor tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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