CTCF Recombinant Rabbit mAb (S-1822-42)

Transcriptional repressor CTCF,11-zinc finger protein,CCCTC-binding factor,CTCFL paralog

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1203

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

CTCF
分子别名

Transcriptional repressor CTCF; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog
免疫原

Synthetic Peptide
细胞定位

Nucleus, Chromosome
Accession

P49711
克隆号

S-1822-42
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

293T, HeLa, K562, MCF7, NIH/3T3, RAW264.7, C6
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:50-1:1000 Hu, Ms, Rt
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:50-1:1000	Hu, Ms, Rt
ChIP	1:20-1:50	Hu

背景介绍

CTCF is a zinc finger protein that binds to specific DNA sequences known as CTCF-binding sites. It functions as a transcriptional regulator, chromatin organizer, and enhancer blocker. CTCF can either activate or repress gene expression, depending on the context and interacting proteins. It can bind to promoters, enhancers, and silencers to regulate transcription. CTCF is involved in the formation of chromatin loops and topologically associating domains (TADs). These loops and domains help organize the genome into functional units and regulate gene expression. CTCF can block the interaction between enhancers and promoters, preventing enhancer-mediated gene activation. This function is important for maintaining tissue-specific gene expression patterns. Mutations or abnormal expression of CTCF have been linked to various diseases, including cancer, developmental disorders, and neurological diseases.

免疫印迹
- WB result of CTCF Recombinant Rabbit mAb
  Primary antibody: CTCF Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: 293T whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: K562 whole cell lysate 20 µg
  Lane 4: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 83 kDa
  Observed MW: 140 kDa
- WB result of CTCF Recombinant Rabbit mAb
  Primary antibody: CTCF Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: RAW264.7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 83 kDa
  Observed MW: 140 kDa
- WB result of CTCF Recombinant Rabbit mAb
  Primary antibody: CTCF Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 83 kDa
  Observed MW: 140 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-CTCF antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CTCF antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CTCF antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CTCF antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-CTCF antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-CTCF antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross – linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
  Parallel reactions used CTCF Recombinant Rabbit mAb (S-1822-42) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for
  immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of INS-Igf2, MYC and SAT-α in
  CTCF Recombinant Rabbit mAb (S-1822-42)-
  immunoprecipitated sample.
组织表达图谱
- Expression of CTCF in tumor tissue.
- Expression of CTCF in human tissue.
- Expression of CTCF in mouse & rat tissue.