CDK5 Recombinant Rabbit mAb (S-1309-115)
Cyclin-dependent kinase 5,Cell division protein kinase 5,Cyclin-dependent-like kinase 5,Serine/threonine-protein kinase PSSALRE,Tau protein kinase II catalytic subunit (TPKII catalytic subunit),CDKN5,PSSALRE
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宿主来源
Rabbit抗原名称
CDK5分子别名
Cyclin-dependent kinase 5; Cell division protein kinase 5; Cyclin-dependent-like kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit (TPKII catalytic subunit); CDKN5; PSSALRE免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
Q00535克隆号
S-1309-115抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, SH-SY5Y, Jurkat, NIH/3T3, Neuro-2a, mouse cerebellum, PC-12, rat cerebellum预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IP 1:50 Hu
CDK5 is a proline-directed serine/threonine kinase that plays a regulatory role in the phosphorylation/dephosphorylation of the eukaryotic cell cycle. Unlike other cyclin-dependent kinases, CDK5 is not activated by cyclins but by p35/p25 proteins, which are highly expressed in the nervous system. CDK5 controls key neural functions such as cytoskeletal structure and dynamics, axonal guidance, neuronal migration, cell adhesion, synaptic transmission, and membrane transport in the nervous system. The absence or dysfunction of CDK5 can lead to neurodevelopmental disorders, while its overactivation can lead to neuronal death and is considered a key factor in Alzheimer's disease. CDK5 and its activator p35 are also expressed in most non-neural tissues, regulating various physiological functions, including hematopoietic cell differentiation, immune response, lymphatic valve formation, myogenesis, melanogenesis, insulin levels, cell migration, wound healing, invasion, survival, and angiogenesis. Abnormal activity of CDK5 is closely related to the pathological changes of various neurodegenerative diseases, including Alzheimer's disease. The role of CDK5 in cancer is also increasingly being studied, with abnormal expression or activation of its activators (especially p35) found in various human cancers, including colorectal, breast, lung, ovarian, prostate, pancreatic, melanoma, and hematological malignancies.
免疫印迹
WB result of CDK5 Recombinant Rabbit mAb
Primary antibody: CDK5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 33 kDa
Observed MW: 30 kDaWB result of CDK5 Recombinant Rabbit mAb
Primary antibody: CDK5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Lane 3: mouse cerebellum lysate lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 33 kDa
Observed MW: 30 kDaWB result of CDK5 Recombinant Rabbit mAb
Primary antibody: CDK5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat cerebellum lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 33 kDa
Observed MW: 30 kDa
免疫沉淀
CDK5 Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CDK5 in 0.4 mg SH-SY5Y whole cell lysate.
Western blot was performed on the immunoprecipitate using CDK5 Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: SH-SY5Y whole cell lysate 40 µg (Input)
Lane 2: CDK5 Recombinant Rabbit mAb IP in SH-SY5Y whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in SH-SY5Y whole cell lysate
Predicted MW: 33 kDa
Observed MW: 30 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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