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Rabbit抗原名称
SUZ12分子别名
Polycomb protein SUZ12; Chromatin precipitated E2F target 9 protein (ChET 9 protein); Joined to JAZF1 protein; Suppressor of zeste 12 protein homolog; CHET9; JJAZ1; KIAA0160免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q15022克隆号
S-1689-3抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, MCF7, Caco-2, NIH/3T3, C6纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
ChIP
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt ICC 1:500 Hu, Ms ICFCM 1:50 Ms ChIP 1:20-1:50 Hu
SUZ12, also known as Zeste 12 Homolog, is a protein encoded by the human SUZ12 gene and is a component of the Polycomb Repressive Complex 2 (PRC2). SUZ12 plays a significant role in epigenetic modification, particularly in Regulating tyrosine trimethylation at position 27 of histone H3. SUZ12 has been implicated in the proliferation and metastasis of various types of cancer. It is overexpressed in tumors such as lung, stomach, colorectal, and breast cancer, where it promotes tumor growth and invasion. In hepatocellular carcinoma, SUZ12 downregulation has been associated with the upregulation of tumor stem cell marker genes, suggesting its importance in tumorigenesis and cancer progression.
免疫印迹
WB result of SUZ12 Recombinant Rabbit mAb
Primary antibody: CTCF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 83 kDa
Observed MW: 90 kDaWB result of SUZ12 Recombinant Rabbit mAb
Primary antibody: CTCF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 83 kDa
Observed MW: 90 kDaWB result of SUZ12 Recombinant Rabbit mAb
Primary antibody: CTCF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 83 kDa
Observed MW: 90 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling SUZ12 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- SUZ12 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- SUZ12 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
Parallel reactionsused SUZ12 Recombinant Rabbit mAb (S-1689-3) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of HOXA1 and SAT-α in SUZ12 Recombinant Rabbit
mAb (S-1689-3)-immunoprecipitated sample.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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