AMPK α1 Rabbit Polyclonal Antibody
5'-AMP-activated protein kinase catalytic subunit alpha-1,AMPK subunit alpha-1,Acetyl-CoA carboxylase kinase (ACACA kinase),Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase),Tau-protein kinase PRKAA1,PRKAA1,AMPK1
货号： S0B1199
- 价格： ￥600
- 规格：
- 数量：
大包装询价 产品介绍 评论(0)
宿主来源
Rabbit抗原名称
AMPK α1分子别名
5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase (ACACA kinase); Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase); Tau-protein kinase PRKAA1; PRKAA1; AMPK1免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
Q13131抗体类型
Polyclonal antibody抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, NIH/3T3, C6预测反应种属
(反应种属缩写表)Or, Pg纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
5' AMP-activated protein kinase or AMPK or 5' adenosine monophosphate-activated protein kinase is an enzyme that plays a role in cellular energy homeostasis, largely to activate glucose and fatty acid uptake and oxidation when cellular energy is low. It belongs to a highly conserved eukaryotic protein family and its orthologues are SNF1 in yeast, and SnRK1 in plants. It consists of three proteins (subunits) that together make a functional enzyme, conserved from yeast to humans. It is expressed in a number of tissues, including the liver, brain, and skeletal muscle. In response to binding AMP and ADP, the net effect of AMPK activation is stimulation of hepatic fatty acid oxidation, ketogenesis, stimulation of skeletal muscle fatty acid oxidation and glucose uptake, inhibition of cholesterol synthesis, lipogenesis, and triglyceride synthesis, inhibition of adipocyte lipogenesis, inhibition of adipocyte lipolysis, and modulation of insulin secretion by pancreatic β-cells.
免疫印迹
WB result of AMPK α1 Rabbit pAb
Primary antibody: AMPK α1 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 64 kDa
Observed MW: 62 kDaWB result of AMPK α1 Rabbit pAb
Primary antibody: AMPK α1 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 64 kDa
Observed MW: 62 kDaWB result of AMPK α1 Rabbit pAb
Primary antibody: AMPK α1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 64 kDa
Observed MW: 62 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-AMPK α1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-AMPK α1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-AMPK α1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-AMPK α1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- AMPK α1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of AMPK α1 in tumor tissue.
Expression of AMPK α1 in human tissue.
Expression of AMPK α1 in mouse & rat tissue.
评论(0)