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Rabbit抗原名称
VIP分子别名
Vasoactive intestinal peptide免疫原
Synthetic Peptide细胞定位
SecretedAccession
P01282克隆号
S-1116-104抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Rt预测反应种属
(反应种属缩写表)Bv, Ms, Pg, Rb, Dg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
IF ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:500 Hu, Rt IF 1:100 Ms
Vasoactive intestinal peptide (VIP) is a neuropeptide that functions as both a neuromodulator and neurotransmitter, with a range of physiological and pathological effects on various bodily systems. VIP receptors are found in various tissues, including the gastrointestinal system, pancreas, pituitary, and liver, mediating the physiological effects of VIP. VIP acts through specific high-affinity receptors, which are G protein-coupled receptors (GPCRs). These receptors, known as VIP receptors or VIPRs, share significant sequence homology and are distinct from the rhodopsin/beta-adrenergic family of receptors. It is a potent vasodilator and plays a role in regulating smooth muscle activity, epithelial cell secretion, and blood flow in the gastrointestinal tract. It stimulates intestinal water and electrolyte secretion, vasodilation, liver glycogenolysis, pancreatic secretion of fluid and enzymes, pituitary prolactin secretion, and smooth muscle relaxation. VIP has been shown to be therapeutic in several inflammatory models, including colitis, sepsis, and collagen-induced arthritis, and has demonstrated immunoregulatory effects in patients with pulmonary sarcoidosis. It may also play a role in wound healing and restoration of immune homeostasis in the cornea following infection.
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-VIP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-VIP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse colon. Anti-VIP antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of VIP in human tissue.
Expression of VIP in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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