热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 其他一抗

所有图片（1/2）
申请试用

Tau (phospho T212/S214) Recombinant Rabbit mAb (S-1538-74)

Microtubule-associated protein tau,Neurofibrillary tangle protein,Paired helical filament-tau (PHF-tau),MAPT,MAPTL,MTBT1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1195
规格
数量
收藏 分享

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Tau (phospho T212/S214)

  • 分子别名

    Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau (PHF-tau); MAPT; MAPTL; MTBT1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm

  • Accession

    P10636-8

  • 克隆号

    S-1538-74

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Ms, Rt

  • 预测反应种属
    (反应种属缩写表)

    Theoretically reacts with most common species

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:1000 Hu, Ms, Rt
背景介绍

  • Tau (phospho T212/S214) refers to the phosphorylation of the tau protein at threonine 212 and serine 214 residues. Phosphorylation at T212 and S214 is associated with the formation of neurofibrillary tangles (NFTs), a hallmark of Alzheimer's disease and other tauopathies. Phosphorylation at T212 and S214 affects the interaction of Tau with DNA and nucleosomes. This modification can reduce its DNA binding affinity and disrupt its phase separation with 12mer arrays, which may have implications for tau's biological role in the nucleus. The levels of phosphorylated tau at T212/S214 increase with healthy aging, and Alzheimer's progression correlates with an increase of nuclear tau phosphorylated at S202/T205 (AT8 antibody epitope), culminating with the exit of tau from the nucleus.

  • 免疫印迹

    • WB result of Tau (phospho T212/S214) Recombinant Rabbit mAb
      Primary antibody: Tau (phospho T212/S214) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: 293T transfected with myc-His-tag, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
      Lane 2: 293T transfected with myc-His-tag, then treated with 100 nM Calyculin A for 30 minutes, then treated with phosphatase whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 79 kDa
      Observed MW: 35~100 kDa

  • 斑点杂交

    • Dot blot result of Tau (phospho T212/S214) Recombinant Rabbit mAb
      Lane1: Tau (Thr212&Ser214) phospho peptide
      Lane2: Tau (Thr212) phospho peptide
      Lane3: Tau (Ser214) phospho peptide
      Lane4: Tau (Thr212&Ser214) unmodified peptide
      Primary antibody: Tau (phospho T212/S214) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

评论(0)