申请试用 
分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
Tau (phospho S396)分子别名
Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau (PHF-tau); MAPT; MAPTL; MTBT1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P10636-8克隆号
S-1016-38抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms阳性样本
Mouse brain预测反应种属
(反应种属缩写表)Theoretically reacts with most common species纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Ms
Tau (phospho S396) refers to the phosphorylation of the tau protein at serine residue 396. Phosphorylation at S396 is associated with the physiological role of tau in synaptic plasticity, which is the dynamic modulation of synaptic connections and is thought to underlie learning and memory processes. Tau phosphorylation at S396 has been shown to regulate the interaction between protein kinase C binding protein 1 (PICK1) and GluA2, which is fundamental to AMPAR internalization, a key molecular mechanism of LTD. Phosphorylation at S396 also contributes to reduced tau-microtubule binding and is correlated with C-terminal tau cleavage and distinct conformational changes in the protein.
免疫印迹
WB result of Tau (phospho S396) Recombinant Rabbit mAb
Primary antibody: Tau (phospho S396) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 5 µg
Lane 2: phosphatase treated mouse brain lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 50~100 kDa
斑点杂交
Dot blot result of Tau (phospho S396) Recombinant Rabbit mAb
Lane 1: Tau (S396) phospho peptide
Lane 2: Tau (S396) phospho peptide
Lane 2: Tau unmodified peptide
Primary antibody: Tau (phospho S396) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
评论(0)