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Rabbit抗原名称
SMAD2分子别名
Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2 (hMAD-2); SMAD family member 2; MADH2; MADR2免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q15796克隆号
S-1420-20抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HT-1080, NIH/3T3, mouse brain, C6, rat brain预测反应种属
(反应种属缩写表)Bv, Or纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IP 1:50 Hu IHC-P 1:100-1:200 Hu, Rt ICC 1:500 Hu ChIP 1:20 Hu
SMAD2 is a key intracellular signaling protein that plays a central role in the canonical transforming growth factor-beta (TGF-β) signaling pathway. SMAD2 is activated when TGF-β superfamily agonists bind to their receptors, leading to the phosphorylation of SMAD2. Once phosphorylated, SMAD2 forms a complex with SMAD4 and translocates into the nucleus to regulate gene expression1. SMAD2 is involved in a variety of cellular processes, including cell proliferation, differentiation, and apoptosis. It is also crucial in developmental processes, particularly in the patterning of the early embryo and the formation of the three germ layers. SMAD2-deficient mice exhibit early embryonic lethality, highlighting the essential role of SMAD2 in development. In the context of adult tissues and disease, SMAD2 has been implicated in the pathogenesis of various cancers, fibrosis, and cardiovascular diseases. It is often dysregulated in cancer, contributing to tumor progression and metastasis. In the heart, SMAD2 signaling has been shown to be important for cardiac development and responses to injury, such as myocardial infarction.
免疫印迹
WB result of SMAD2 Recombinant Rabbit mAb
Primary antibody: SMAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HT-1080 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 52 kDa
Observed MW: 60 kDaWB result of SMAD2 Recombinant Rabbit mAb
Primary antibody: SMAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 52 kDa
Observed MW: 60 kDaWB result of SMAD2 Recombinant Rabbit mAb
Primary antibody: SMAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 52 kDa
Observed MW: 60 kDa
免疫沉淀
SMAD2 Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating SMAD2 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using SMAD2 Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 µg (Input)
Lane 2: SMAD2 Recombinant Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 52 kDa
Observed MW: 60 kDa
Exposure time: 38 s
免疫组化
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-SMAD2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-SMAD2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-SMAD2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- SMAD2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HaCaT + TGF-β (7ng/mL, 1h) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used SMAD2 Recombinant Rabbit mAb (S-1420-20) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:20 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of ID1, CDKN1A and SAT-α in
SMAD2 Recombinant Rabbit mAb (S-1420-20)-immunoprecipitated
sample.
组织表达图谱
Expression of SMAD2 in tumor tissue.
Expression of SMAD2 in human tissue.
Expression of SMAD2 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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