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Rabbit抗原名称
RPA70分子别名
Replication protein A 70 kDa DNA-binding subunit; RP-A p70; Replication factor A protein 1 (RF-A protein 1); Single-stranded DNA-binding protein; RPA1; REPA1免疫原
Synthetic Peptide细胞定位
NucleusAccession
P27694克隆号
S-1653-9抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Rt阳性样本
HEK-293, K562, HeLa, C6预测反应种属
(反应种属缩写表)Or纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM ,IHC-P ? ,ICC ? ,WB ,IP稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Rt IP 1:50 Hu IHC-P 1:50 Hu, Rt ICC 1:500 Hu ICFCM 1:500 Hu
RPA70 is part of the heterotrimeric RPA complex, which is the primary single-stranded DNA (ssDNA) binding protein in eukaryotes. It plays a central role in almost all aspects of DNA replication, repair, and recombination. RPA70 is involved in cellular DNA damage response. It can interact with a SUMO-specific protease SENP6 and is modified by SUMO, which is essential for homologous recombination repair of DNA double-strand breaks. RPA70 undergoes phosphorylation, which is important for mediating activation of the ATR checkpoint and maintaining genomic stability. The N-terminal domain of RPA70 has weak DNA binding activity and is involved in protein interactions and cellular DNA damage response. RPA70 and the RPA complex are also considered promising targets for anti-cancer therapeutics. Inhibition of RPA can abrogate ATR activity and decrease the cellular ssDNA protection threshold, making it a potential strategy for single agent or combination treatment in cancer.
免疫印迹
WB result of RPA70 Recombinant Rabbit mAb
Primary antibody: RPA70 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 68 kDa
Observed MW: 68 kDaWB result of RPA70 Recombinant Rabbit mAb
Primary antibody: RPA70 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 68 kDa
Observed MW: 68 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling RPA70 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
RPA70 Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating RPA70 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using RPA70 Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 µg (Input)
Lane 2: RPA70 Recombinant Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 68 kDa
Observed MW: 68 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-RPA70 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-RPA70 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-RPA70 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-RPA70 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-RPA70 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- RPA70 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of RPA70 in tumor tissue.
Expression of RPA70 in human tissue.
Expression of RPA70 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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