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Rabbit抗原名称
Phospho-Tuberin (T1462)分子别名
Tuberous sclerosis 2 protein; TSC2; TSC4免疫原
Synthetic Peptide细胞定位
Cytoplasm, LysosomeAccession
P49815克隆号
S-987-126抗体类型
Recombinant mAb抗体同种型
IgG应用
WB反应种属 ?
Hu纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:250-1:1000 | Hu |
Phospho-Tuberin (T1462) refers to the phosphorylation of Tuberin, also known as TSC2 (Tuberous Sclerosis Complex 2), at the threonine residue 1462. This post-translational modification is significant in the regulation of the Tuberous Sclerosis Complex, which plays a crucial role in cellular energy response pathways. Tuberin is a tumor suppressor that forms a complex with TSC1, inhibiting the mTORC1 pathway, which is a central nutrient sensor promoting cellular growth and biomass generation. Phosphorylation at T1462 is one of the modifications that can affect Tuberin's function and its interaction with TSC1. This phosphorylation event is stimulated by various growth factors and is mediated through the phosphoinositide 3-kinase/Akt pathway.
免疫印迹
WB result of Phospho-Tuberin (T1462) Recombinant Rabbit mAb
Primary antibody: Phospho-Tuberin (T1462) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated SH-SY5Y whole cell lysate 20 µg
Lane 2: SH-SY5Y treated with 1 µM Okadaic Acid for 60 minutes and 200 nM Calyculin A for 60 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 200 kDa
Observed MW: 220 kDa
This blot was developed with high sensitivity substrate
斑点杂交
Dot blot result of Phospho-Tuberin (T1462) Recombinant Rabbit mAb
Lane 1: Tuberin (T1462) phospho peptide
Lane 2: Tuberin unmodified peptide
Primary antibody: Phospho-Tuberin (T1462) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
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