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Rabbit抗原名称
Phospho-PDHA1 (S293)分子别名
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; PHE1A免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
P08559克隆号
S-1729-25抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms, Rt阳性样本
HepG2, HEK-293, HeLa, NIH/3T3, mouse brain, PC-12, Rat brain预测反应种属
(反应种属缩写表)Bv, CyMk, Or, Pg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
PDHA1, or pyruvate dehydrogenase E1 subunit alpha 1, is a crucial component of the pyruvate dehydrogenase complex (PDHc), which is a mitochondrial multienzyme complex that catalyzes the conversion of pyruvate to acetyl-CoA and CO2, providing a primary link between glycolysis and the tricarboxylic acid (TCA) cycle. Phosphorylation of PDHA1 at Ser293 (PDHA1 phospho S293) is an important regulatory modification that affects the activity of the PDH complex, which is crucial for the regulation of glucose metabolism and fatty acid oxidation, which are sensitive targets for insulin action.
免疫印迹
WB result of phospho-PDHA1 (S293) Recombinant Rabbit mAb
Primary antibody: phospho-PDHA1 (S293) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 43 kDa
Observed MW: 43 kDaWB result of phospho-PDHA1 (S293) Recombinant Rabbit mAb
Primary antibody: phospho-PDHA1 (S293) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: phosphatase treated HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 43 kDa
Observed MW: 43 kDaWB result of phospho-PDHA1 (S293) Recombinant Rabbit mAb
Primary antibody: phospho-PDHA1 (S293) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 43 kDa
Observed MW: 43 kDaWB result of phospho-PDHA1 (S293) Recombinant Rabbit mAb
Primary antibody: phospho-PDHA1 (S293) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 43 kDa
Observed MW: 43 kDa
斑点杂交
Dot blot result of phospho-PDHA1 (S293) Recombinant Rabbit mAb
Lane 1: PDHA1 (S293) phospho peptide
Lane 2: PDHA1 unmodified peptide
Primary antibody: phospho-PDHA1 (S293) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma and negative staining in cervical squamous cell carcinoma treated with phosphatase. Anti- Phospho-PDHA1 (S293) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney and negative staining in human kidney treated with phosphatase. Anti- Phospho-PDHA1 (S293) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach and negative staining in mouse stomach treated with phosphatase. Anti- Phospho-PDHA1 (S293) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle and negative staining in rat cardiac muscle treated with phosphatase. Anti- Phospho-PDHA1 (S293) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Phospho-PDHA1 (S293) in mouse & rat tissue.
Expression of Phospho-PDHA1 (S293) in tumor tissue.
Expression of Phospho-PDHA1 (S293) in human tissue.
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