Phospho-GCN2 (T899) Recombinant Rabbit mAb (S-1665-19)

eIF-2-alpha kinase GCN2,Eukaryotic translation initiation factor 2-alpha kinase 4,GCN2-like protein,EIF2AK4,KIAA1338

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1186

规格

25μl100μl

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-GCN2 (T899)
分子别名

eIF-2-alpha kinase GCN2; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2-like protein; EIF2AK4; KIAA1338
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

Q9P2K8
克隆号

S-1665-19
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Ms
IHC-P 1:200 Rt

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Ms
IHC-P	1:200	Rt

背景介绍

GCN2, or General Control Nonderepressible-2, is a kinase belonging to the protein kinase family. It is known for its ability to phosphorylate the alpha subunit of the eukaryotic translation initiation factor 2 (eIF2α), thereby downregulating protein synthesis under various cellular stress conditions. GCN2 is activated in response to amino acid deprivation and binds to uncharged tRNAs, and it may also be activated under conditions of glucose deprivation and viral infections. Phosphorylated GCN2 (phospho T899) is a key regulatory point for the kinase activity of GCN2. When GCN2 is phosphorylated at the Thr899 site, it can more effectively phosphorylate Ser51 of eIF2α, which in turn inhibits global protein translation rates and reduces the consumption of amino acids within the cell. This mechanism makes GCN2 an important sensor and regulator in the cellular response to amino acid starvation and other stress conditions. In the tumor microenvironment, the activation of GCN2 is associated with the modulation of immune responses. Studies have shown that the activation of GCN2 can lead to T cell anergy and apoptosis, enhance myeloid-derived suppressor cell (MDSC)-dependent immunosuppression, and promote tumor cell survival. Therefore, the inhibition of GCN2 may have direct anti-cancer effects and immune-activating effects, making it a potential therapeutic target for cancer treatment.

免疫印迹
- WB result of phospho-GCN2 (T899) Recombinant Rabbit mAb
  Primary antibody: phospho-GCN2 (T899) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 starve 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 187 kDa
  Observed MW: 220 kDa
斑点杂交
- Dot blot result of phospho-GCN2 (T899) Recombinant Rabbit mAb
  Lane 1: GCN2 (T899) phospho peptide
  Lane 2: GCN2 unmodified peptide
  Primary antibody: phospho-GCN2 (T899) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilutio
免疫组化
- IHC shows positive staining in paraffin-embedded mouse liver and negative staining in mouse liver treated with phosphatase. Anti- Phospho-GCN2 (T899) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney and negative staining in rat kidney treated with phosphatase. Anti- Phospho-GCN2 (T899) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis and negative staining in rat testis treated with phosphatase. Anti- Phospho-GCN2 (T899) antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of Phospho-GCN2 (T899) in mouse & rat tissue.