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宿主来源
Rabbit抗原名称
Peroxiredoxin 3分子别名
Thioredoxin-dependent peroxide reductase, mitochondrial; Antioxidant protein 1 (AOP-1); HBC189; Peroxiredoxin III (Prx-III); Protein MER5 homolog; Thioredoxin-dependent peroxiredoxin 3; PRDX3; AOP1免疫原
Synthetic Peptide细胞定位
Cytoplasm, MitochondrionAccession
P30048抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, MCF7, A431, mouse heart, rat liver预测反应种属
(反应种属缩写表)Bv, Or纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu, Ms, Rt
Peroxiredoxin 3 (PRDX3) is a mitochondrial antioxidant enzyme that plays a significant role in maintaining cellular redox balance and protecting cells from oxidative stress. PRDX3 is known to be upregulated during tumorigenesis and cancer progression. It regulates cellular signaling pathways associated with Matrix Metalloproteinase-1 (MMP-1) expression and activity in breast cancer progression. Silencing PRDX3 inhibits cell migration and invasion in triple-negative breast cancer cell lines, while overexpression promotes these processes, which are important in the metastatic cascade. PRDX3 is crucial in the regulation of mitochondrial homeostasis and macrophage polarization. A deficiency of PRDX3 leads to an increase in mitochondrial superoxide levels and the induction of inflammation. PRDX3 is also a mitochondrial hydrogen peroxide scavenger that protects cancer cells against hypoxia and drug-induced hydrogen peroxide-dependent apoptosis. In addition, PRDX3 plays a protective role against mitochondrial dysfunction by removing mitochondrial reactive oxygen species.
免疫印迹
WB result of Peroxiredoxin 3 Rabbit pAb
Primary antibody: Peroxiredoxin 3 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 28 kDa
Observed MW: 22~25 kDaWB result of Peroxiredoxin 3 Rabbit pAb
Primary antibody: Peroxiredoxin 3 Rabbit pAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 28 kDa
Observed MW: 22~25 kDaWB result of Peroxiredoxin 3 Rabbit pAb
Primary antibody: Peroxiredoxin 3 Rabbit pAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 28 kDa
Observed MW: 23 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Peroxiredoxin 3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Peroxiredoxin 3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Peroxiredoxin 3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Peroxiredoxin 3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Peroxiredoxin 3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Peroxiredoxin 3 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Peroxiredoxin 3 in tumor tissue.
Expression of Peroxiredoxin 3 in human tissue.
Expression of Peroxiredoxin 3 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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