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Rabbit抗原名称
NQO1分子别名
NAD(P)H dehydrogenase [quinone] 1; Azoreductase; DT-diaphorase 1 (DTD); Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1 (QR1); DIA4; NMOR1免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P15559克隆号
S-1672-2抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HepG2, HeLa, MCF7预测反应种属
(反应种属缩写表)Or纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:20000 Hu IHC-P 1:100 Hu ICC 1:100 Hu ICFCM 1:50 Hu
NQO1, or NAD(P)H:quinone oxidoreductase 1, is an enzyme encoded by the NQO1 gene in humans. It is a protective antioxidant and a multifunctional cell protector that can modulate the oxidative stress response to DNA damage in cancer cells by binding to chromatin-binding proteins. NQO1 is a homodimeric flavoenzyme that catalyzes the two-electron reduction of quinones to hydroquinones, thereby facilitating the excretion of quinones that would otherwise form semiquinones and generate reactive oxygen species (ROS) through redox cycling. NQO1 plays a crucial role in preventing oxidative damage to DNA caused by environmental stressors and maintains the reduced forms of endogenous antioxidants such as ubiquinone and α-tocopherol quinone. It is highly expressed in many solid tumors, including non-small cell lung cancer, pancreatic cancer, breast cancer, prostate cancer, colon cancer, and head and neck cancer, while its expression is very low in normal cells/tissues, making it an ideal target for cancer therapy. NQO1 has also been implicated in the regulation of glucose and insulin metabolism, which is relevant to diabetes and metabolic syndrome, as well as in Alzheimer's disease and aging.
免疫印迹
WB result of NQO1 Rabbit pAb
Primary antibody: NQO1 Rabbit pAb at 1/20000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 31 kDa
Observed MW: 30 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling NQO1 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-NQO1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-NQO1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-NQO1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- NQO1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of NQO1 in tumor tissue.
Expression of NQO1 in human tissue.
Expression of NQO1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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