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Rabbit抗原名称
GABRD分子别名
Gamma-aminobutyric acid receptor subunit delta; GABA(A) receptor subunit delta (GABAAR subunit delta)免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P22933克隆号
S-1584-162抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
mouse brain, mouse cerebellum, rat brain, rat cerebellum纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:200-1:500 Hu, Ms, Rt
GABRD is the delta subunit of the GABA (Gamma-Aminobutyric Acid) A-type receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain, and its action on GABA-A receptors can be modulated by various drugs such as barbiturates, benzodiazepines, steroids, or alcohol. The role of GABRD in various cancers has been studied. For instance, in Colon Adenocarcinoma (COAD), the expression of GABRD is significantly elevated and associated with advanced clinical staging. High GABRD expression correlates with shorter overall survival and progression-free survival in patients, making it an independent predictor of overall survival. In Breast Cancer, GABRD promotes breast cancer progression through CDK1-dependent cell cycle regulation, with significantly elevated expression levels in tumor tissues and correlation with advanced tumor progression. Additionally, GABRD is considered prognostic in Bladder Urothelial Carcinoma and Clear Cell Renal Cell Carcinoma. In Gastric Cancer (GC), high expression levels of GABRD mRNA are associated with poor prognosis. The function of GABRD in proliferation, invasion, and apoptosis experiments has been assessed, indicating that GABRD plays a significant role in GC cell proliferation and invasion and is correlated with poor prognosis, suggesting that GABRD may be a potential therapeutic target for gastric cancer.
免疫印迹
WB result of GABRD Recombinant Rabbit mAb
Primary antibody: GABRD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse kidney lysate 20 µg
Lane 2: unboiled mouse brain lysate 20 µg
Lane 3: unboiled mouse cerebellum lysate 20 µg
Negative control: unboiled mouse kidney lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 51 kDa
Observed MW: 51 kDaWB result of GABRD Recombinant Rabbit mAb
Primary antibody: GABRD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat kidney lysate 20 µg
Lane 2: unboiled rat brain lysate 20 µg
Lane 3: unboiled rat cerebellum lysate 20 µg
Negative control: unboiled rat kidney lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 51 kDa
Observed MW: 51 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebellum. Anti-GABRD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-GABRD antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GABRD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GABRD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat kidney. Anti-GABRD antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of GABRD in tumor tissue.
Expression of GABRD in human tissue.
Expression of GABRD in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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