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Rabbit抗原名称
DFNA5/GSDME分子别名
Gasdermin-E; Inversely correlated with estrogen receptor expression 1 (ICERE-1); Non-syndromic hearing impairment protein 5; ICERE1免疫原
Synthetic PeptideAccession
O60443克隆号
S-1703-1抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
SH-SY5Y, HeLa纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ? ,WB ,IP稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IP 1:50:00 IHC-P 1:250 Hu
GSDME (Gasdermin E), also known as DFNA5 or ICERE-1, is a protein found in human cells and is a member of the Gasdermin family. GSDME has membrane pore-forming activity, and its primary mechanism of action involves being activated by Caspase 3 cleavage, which induces a switch from apoptosis to pyroptosis in cells. GSDME is associated with the development of neurodegenerative diseases, and its expression in cochlear hair cells is related to the progression of sensorineural hearing loss. GSDME plays a role in regulating the inflammatory response, and its activation can lead to the release of inflammatory factors, such as IL-1β and IL-18. GSDME is also a potential target in cancer immunotherapy. It is highly expressed in normal tissues but has low expression in certain cancer tissues, which may be related to its role in tumor suppression. The expression level of GSDME can affect the efficacy of chemotherapeutic drugs, and its absence may reduce the effectiveness of some chemotherapy drugs.
免疫印迹
WB result of DFNA5/GSDME Recombinant Rabbit mAb
Primary antibody: DFNA5/GSDME Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Weak expression: LNCaP whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55 kDa
Observed MW: 55 kDa
免疫沉淀
DFNA5/GSDME Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating DFNA5/GSDME in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using DFNA5/GSDME Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: DFNA5/GSDME Recombinant Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 55 kDa
Observed MW: 55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-DFNA5/GSDME antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-DFNA5/GSDME antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-DFNA5/GSDME antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of DFNA5/GSDME in tumor tissue.
Expression of DFNA5/GSDME in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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