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Rabbit抗原名称
Ctip2分子别名
B-cell lymphoma/leukemia 11B; BCL-11B; B-cell CLL/lymphoma 11B; COUP-TF-interacting protein 2; Radiation-induced tumor suppressor gene 1 protein (hRit1); RIT1; BCL11B免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q9C0K0克隆号
S-1375-21抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Molt-4, Jurkat纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM ,IHC-P ? ,WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:100-1:200 Hu, Ms, Rt ICFCM 1:500 Hu
Ctip2, also known as COUP-TF interacting protein 2 or BCL11B, is a multifunctional transcription factor involved in numerous cellular physiological processes. It plays a significant role in skin development and maintenance, particularly in the regulation of the epidermal permeability barrier (EPB), wound healing, inflammatory diseases, and epithelial cancers. It also has a relatively unexplored connection with lipid metabolism control and its impact on EPB homeostasis, delayed wound healing, exacerbation of inflammatory diseases, and promotion and progression of cancer. Ctip2 controls keratinocyte proliferation and cytoskeletal organization, and regulates the onset and maintenance of differentiation in keratinocytes. It integrates keratinocyte proliferation and the switch to differentiation by directly and positively regulating EGFR transcription in proliferating cells and Notch1 transcription in differentiating cells. Ctip2 is known for its role in DNA double-strand break (DSB) repair and genome stability. It is an interacting partner of the Mre11/Rad50/Nbs1 (MRN) DNA damage sensor protein complex, which recognizes DNA double-strand breaks and promotes the resection of 5′ strands to generate 3′ single-stranded intermediates necessary for homologous recombination. Ctip2 is central to the differentiation of medium spiny neurons (MSN) and striatal development. In the absence of Ctip2, MSN do not fully differentiate, leading to severely disrupted patch-matrix organization within the striatum, which is critical in motor control and affected in diseases like Huntington's. Ctip2 is also involved in the formation of T lymphocytes, which is crucial for the immune system.
免疫印迹
WB result of Ctip2 Recombinant Rabbit mAb
Primary antibody: Ctip2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: Molt-4 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Negative control: Raji whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 96 kDa
Observed MW: 115 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Raji (Human Burkitt's lymphoma B lymphocyte, Left) / Jurkat (Human T cell leukemia T lymphocyte, Right) labelling Ctip2 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: Raji
免疫组化
IHC shows positive staining in paraffin-embedded human thymus. Anti-Ctip2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Ctip2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Ctip2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Ctip2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-Ctip2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Ctip2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-Ctip2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Ctip2 in tumor tissue.
Expression of Ctip2 in human tissue.
Expression of Ctip2 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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