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Rabbit抗原名称
COX1/Cyclooxygenase 1分子别名
Prostaglandin G/H synthase 1; Prostaglandin H2 synthase 1 (PGH synthase 1; PGHS-1; PHS 1); Prostaglandin-endoperoxide synthase 1; PTGS1免疫原
Synthetic Peptide细胞定位
Endoplasmic reticulum membraneAccession
P23219克隆号
S-1478-48抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
A431, HeLa, HaCaT, C2C12, NIH/3T3, mouse cerebellum, mouse kidney, rat cerebellum, rat kidney纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IP 1:50 IHC-P 1:250 Hu, Ms, Rt
COX1, full name Cyclooxygenase-1, is one of the two isoforms of cyclooxygenase (COX), with the other being COX-2. COX1 is a constitutive enzyme that is widely expressed in most tissues such as blood vessels, stomach, kidneys, and platelets. It is involved in platelet aggregation, vascular tone, gastric mucosal blood flow, and regulation of renal blood flow, helping to maintain the stability of cellular, tissue, and organ physiological functions. Compared to COX-2, COX1 is constitutively expressed and plays a role in the synthesis of physiological prostaglandins (PGs), which regulate normal tissue cell activities such as protecting the gastrointestinal mucosa and altering vascular tone. COX1 plays a smaller role in inflammatory damage compared to COX-2. Inflammation primarily stimulates monocytes, macrophages, etc., inducing the production of COX-2, which is a key link in triggering subsequent inflammatory responses.
免疫印迹
WB result of COX1/Cyclooxygenase 1 Recombinant Rabbit mAb
Primary antibody: COX1/Cyclooxygenase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HaCaT whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 69 kDa
Observed MW: 58, 70 kDaWB result of COX1/Cyclooxygenase 1 Recombinant Rabbit mAb
Primary antibody: COX1/Cyclooxygenase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: mouse cerebellum lysate 20 µg
Lane 4: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 69 kDa
Observed MW: 58, 70 kDaWB result of COX1/Cyclooxygenase 1 Recombinant Rabbit mAb
Primary antibody: COX1/Cyclooxygenase 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat cerebellum lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 69 kDa
Observed MW: 56, 70 kDa
免疫沉淀
COX1/Cyclooxygenase 1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating COX1/Cyclooxygenase 1 in 0.4 mg NIH/3T3 whole cell lysate.
Western blot was performed on the immunoprecipitate using COX1/Cyclooxygenase 1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: NIH/3T3 whole cell lysate 20 µg (Input)
Lane 2: COX1/Cyclooxygenase 1 Rabbit mAb IP in NIH/3T3 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in NIH/3T3 whole cell lysate
Observed MW: 58, 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human lung. Anti- COX1/Cyclooxygenase 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti- COX1/Cyclooxygenase 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti- COX1/Cyclooxygenase 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti- COX1/Cyclooxygenase 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti- COX1/Cyclooxygenase 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of COX1/Cyclooxygenase 1 in tumor tissue.
Expression of COX1/Cyclooxygenase 1 in human tissue.
Expression of COX1/Cyclooxygenase 1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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