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Clathrin heavy chain Recombinant Rabbit mAb (S-1690-26)

Clathrin heavy chain 1,Clathrin heavy chain on chromosome 17 (CLH-17),CLTC,CLH17,CLTCL2,KIAA0034

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1174
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Clathrin heavy chain

  • 分子别名

    Clathrin heavy chain 1; Clathrin heavy chain on chromosome 17 (CLH-17); CLTC; CLH17; CLTCL2; KIAA0034

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Melanosome

  • Accession

    Q00610

  • 克隆号

    S-1690-26

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    A431, SH-SY5Y, HeLa, 293T, Neuro-2a, NIH/3T3, C6, PC-12

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
背景介绍

  • Clathrin heavy chain (CHC) is a critical component of the clathrin protein complex, which is involved in intracellular trafficking, particularly in clathrin-mediated endocytosis (CME). The major function of neuronal clathrin is to recycle synaptic vesicle proteins following exocytosis. Clathrin heavy chain is probably the limiting component in triskelion biosynthesis, and an increase in the amount of clathrin heavy chain may reflect control of triskelion synthesis. Clathrin-mediated pathways are linked to disease and tissue differentiation. The balance of cargo sequestration into CCVs can affect cell fate, linking clathrin-mediated pathways to disease.

  • 免疫印迹

    • WB result of Clathrin heavy chain Recombinant Rabbit mAb
      Primary antibody: Clathrin heavy chain Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: A431 whole cell lysate 20 µg
      Lane 2: SH-SY5Y whole cell lysate 20 µg
      Lane 3: HeLa whole cell lysate 20 µg
      Lane 4: 293T whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 192 kDa
      Observed MW: 180 kDa

    • WB result of Clathrin heavy chain Recombinant Rabbit mAb
      Primary antibody: Clathrin heavy chain Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: Neuro-2a whole cell lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 192 kDa
      Observed MW: 180 kDa

    • WB result of Clathrin heavy chain Recombinant Rabbit mAb
      Primary antibody: Clathrin heavy chain Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 192 kDa
      Observed MW: 180 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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