大包装询价 产品介绍 评论(0)
宿主来源
Rabbit抗原名称
CD4分子别名
T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P06332克隆号
S-634-260抗体类型
Recombinant mAb抗体同种型
IgG应用
FCM反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1:500 | Ms |
CD4 is a glycoprotein found on the surface of immune cells, primarily known for its role as a coreceptor on T-helper cells, a subset of T lymphocytes. CD4 is used as a marker to distinguish between different T cell subsets, particularly T-helper cells (CD4+ T cells) and T-regulatory cells (also known as Tregs, which can be CD4+CD25+). CD4 acts as a coreceptor with the T cell receptor (TCR) to recognize antigens presented on the surface of antigen-presenting cells (APCs) by MHC class II molecules. This interaction is crucial for the activation of T-helper cells. CD4+ T cells are central to the regulation of immune responses. They help activate B cells, macrophages, and other immune cells, and they produce cytokines that influence the type of immune response (e.g., TH1, TH2, Tfh, Tregs).
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling CD4 antibody at 1/500 (0.1 μg) dilution/ (Right panel) compared with a Rabbit IgG Isotype Control / (Left panel). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD8 - Alexa Fluor® 647 antibody separately.
评论(0)