分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
DCAMKL1分子别名
Serine/threonine-protein kinase DCLK1; Doublecortin domain-containing protein 3A; Doublecortin-like and CAM kinase-like 1; Doublecortin-like kinase 1; DCLK1; DCDC3A; KIAA0369免疫原
Synthetic PeptideAccession
O15075克隆号
S-1705-76抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
SH-SY5Y, TT, NIH/3T3, mouse brain, mouse colon, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Ms, Rt ICC 1:500 Ms
DCAMKL1, or doublecortin and CaM kinase-like 1, is a protein that has homology to doublecortin (DCX) and is involved in regulating microtubule polymerization. It consists of an N-terminal region that is similar to DCX and a C-terminal domain that encodes a putative Ca2+/calmodulin-dependent protein kinase. It is expressed in the central and peripheral nervous systems, particularly in migrating neuronal populations, and is associated with microtubules. DCAMKL1 has been shown to associate with microtubules, stimulate the polymerization of purified tubulin, and form aster-like microtubule structures. Overexpression of DCAMKL1 can lead to microtubule bundling in cell lines and cultured primary neural cells. DCAMKL1 has also been implicated in the regulation of osteoblast function. Studies have shown that knocking down DCAMKL1 in mesenchymal stem cells enhances their differentiation into osteoblasts, and DCAMKL1 gene knockout mice exhibit increased osteoblast function, bone formation rate, and bone density.
免疫印迹
WB result of DCAMKL1 Recombinant Rabbit mAb
Primary antibody: DCAMKL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Lane 2: TT whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 82 kDa
Observed MW: 40~90 kDaWB result of DCAMKL1 Recombinant Rabbit mAb
Primary antibody: DCAMKL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse colon lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 82 kDa
Observed MW: 40~90 kDaWB result of DCAMKL1 Recombinant Rabbit mAb
Primary antibody: DCAMKL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 82 kDa
Observed MW: 40~90 kDa
免疫组化
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-DCAMKL1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-DCAMKL1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in NIH/3T3 cells. Anti- DCAMKL1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of DCAMKL1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)