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Rabbit抗原名称
CD1A分子别名
T-cell surface glycoprotein CD1a; T-cell surface antigen T6/Leu-6 (hTa1 thymocyte antigen)免疫原
Recombinant ProteinAccession
P06126克隆号
S-1486-65抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Molt-4, Jurkat纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ? ,WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu
CD1a is a member of the CD1 family of cell-surface glycoproteins that present lipid antigens to T cells. It is structurally related to MHC class I molecules, but its antigen-binding cleft is specifically designed to accommodate lipids rather than peptides. CD1a is characterized by its small antigen-binding groove and high expression on Langerhans cells in the epidermis. CD1a is involved in the adaptive immune response to many microbial lipid antigens. It is expressed on immature thymocytes and is lost upon T cell maturation. In the skin, CD1a is constitutively highly expressed on Langerhans cells, which are ideally positioned to detect breaches in the skin barrier and changes in the local extracellular milieu. Beyond Langerhans cells, CD1a is expressed at lower levels on a subset of dermal myeloid dendritic cells. In inflammatory skin diseases such as atopic dermatitis and psoriasis, the frequency of CD1a-expressing dendritic cell subsets is increased, and migratory patterns of Langerhans cells are altered.
免疫印迹
WB result of CD1a Recombinant Rabbit mAb
Primary antibody: CD1a Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Molt-4 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 37 kDa
Observed MW: 42 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human esophagus. Anti-CD1a antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD1a antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-CD1a antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CD1a in tumor tissue.
Expression of CD1a in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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