S-RMab^®CD4 Recombinant Rabbit mAb (S-R569)

T-cell surface glycoprotein CD4,T-cell differentiation antigen L3T4,T-cell surface antigen T4/Leu-3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1165

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CD4
分子别名

T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3
细胞定位

Cell membrane
Accession

P06332
克隆号

S-R569
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

IF ?
稀释度

应用稀释度推荐种属

IHC-P 1:250 Ms
IF 1:100 Ms

应用	稀释度	推荐种属
IHC-P	1:250	Ms
IF	1:100	Ms

背景介绍

CD4 is a glycoprotein found on the surface of immune cells, primarily known for its role as a coreceptor on T-helper cells, a subset of T lymphocytes. CD4 is used as a marker to distinguish between different T cell subsets, particularly T-helper cells (CD4+ T cells) and T-regulatory cells (also known as Tregs, which can be CD4+CD25+). CD4 acts as a coreceptor with the T cell receptor (TCR) to recognize antigens presented on the surface of antigen-presenting cells (APCs) by MHC class II molecules. This interaction is crucial for the activation of T-helper cells. CD4+ T cells are central to the regulation of immune responses. They help activate B cells, macrophages, and other immune cells, and they produce cytokines that influence the type of immune response (e.g., TH1, TH2, Tfh, Tregs).

免疫组化
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex (T cells). Anti-CD4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse spleen. Anti-CD4 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded mouse colon. Anti-CD4 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
- Expression of CD4 in mouse & rat tissue.