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Rabbit抗原名称
CD31分子别名
Platelet endothelial cell adhesion molecule; PECAM-1细胞定位
Cell membraneAccession
Q08481克隆号
S-R568抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, IF反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| IHC-P | 1:250 | Ms, Rt, Hu |
| IF | 1:500 | Ms |
CD31, also known as Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1), is a 130 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is widely expressed on the surface of various cells, including endothelial cells, circulating platelets, monocytes, neutrophils, and certain T cell subsets. CD31 plays a crucial role in cell-cell adhesion and signaling, particularly in immune cell transmigration and angiogenesis. It is involved in the clearance of senescent neutrophils and likely plays a role in leukocyte migration, vascular integrity, and the activation of integrins. Activation of CD31 has been linked to signaling events that can trigger both atherogenesis and anti-atherogenic endothelial NO production, suggesting a complex physiology dependent on various factors. CD31 is considered a potential biomarker for the efficacy of bevacizumab in ovarian cancer, as the impact of bevacizumab on progression-free survival and overall survival is most pronounced in patients with high CD31 expression. It is also used in immunohistochemistry to identify endothelial tissues, assess tumor angiogenesis, and diagnose vascular tumors.
免疫组化
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-CD31 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-CD31 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-CD31 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-CD31 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-CD31 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD31 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-CD31 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD31 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse lung. Anti-CD31 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of CD31 in mouse & rat tissue.
Expression of CD31 in tumor tissue.
Expression of CD31 in human tissue.
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