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Rabbit抗原名称
Collagen II分子别名
Collagen alpha-1(II) chain; Alpha-1 type II collagen; COL2A1免疫原
Synthetic Peptide细胞定位
Extracellular space, SecretedAccession
P02458抗体类型
Polyclonal antibody抗体同种型
IgG应用
WB反应种属 ?
Hu阳性样本
TT纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
Collagen II, also known as type II collagen, is a fibrillar collagen that plays a crucial role in the structure and function of various tissues, particularly in cartilage. It is the main fibrillar collagen of growth plate cartilage and other cartilages, accounting for 95% of the collagens and approximately 60% of the dry weight of cartilage. Collagen II is not only found in cartilage but also in the vitreous humor, intervertebral disks, and inner ear. It is important for the normal development of embryonic bone, linear growth, and the ability of cartilage to resist pressure. Mutations in Collagen II can result in several types of chondrodysplasia, leading to premature osteoarthritis. It is also associated with diseases like Stickler syndrome, Wagner syndrome, and Kniest dysplasia, which can affect the vitreous and other connective tissues.
免疫印迹
WB result of Collagen II Rabbit pAb
Primary antibody: Collagen II Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: TT whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 142 kDa
Observed MW: 200 kDa
This blot was developed with high sensitivity substrate
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