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Rabbit抗原名称
Collagen XVII分子别名
Collagen alpha-1(XVII) chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2; COL17A1; BP180; BPAG2免疫原
Synthetic Peptide细胞定位
Membrane, Cell junctionAccession
Q9UMD9克隆号
S-1636-5抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
A431, HaCaT纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IP 1:50 Hu IHC-P 1:500 Hu ICC 1:500 Hu
Collagen XVII, also known as COL17 or BP180, is a transmembrane protein that plays a crucial role in maintaining the adhesion between the epidermis and the basement membrane in the skin. It is a component of hemidesmosomes, which are multiprotein complexes that mediate the adhesion of keratinocytes to the underlying membrane. It is involved in cell-cell adhesion and signaling, particularly in immune cell transmigration and angiogenesis. The extracellular domain of COL17 can be physiologically cleaved from the cell surface by ADAM family proteins in a process known as ectodomain shedding, which is thought to be associated with the migration and proliferation of keratinocytes. The C-terminal cleavage of COL17 may be associated with basement membrane formation. Collagen XVII is involved in skin homeostasis, aging, and wound repair. It mediates the interactions of stem cells with surrounding cells and the matrix to regulate these processes. Changes in COL17 expression and function are associated with skin aging, as it affects the balance of symmetric and asymmetric cell divisions in epidermal stem cells. COL17 has also been implicated in skin cancers, such as squamous cell carcinoma, basal cell carcinoma, and malignant melanocytic tumors.
免疫印迹
WB result of Collagen XVII Recombinant Rabbit mAb
Primary antibody: Collagen XVII Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HaCaT whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 150 kDa
Observed MW: 130 kDa
This blot was developed with high sensitivity substrate
免疫沉淀
Collagen XVII Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Collagen XVII in 0.4 mg A431 whole cell lysate.
Western blot was performed on the immunoprecipitate using Collagen XVII Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: A431 whole cell lysate 40 µg (Input)
Lane 2: Collagen XVII Recombinant Rabbit mAb IP in A431 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A431 whole cell lysate
Predicted MW: 150 kDa
Observed MW: 130 kDa
Exposure time: 30 s
免疫组化
IHC shows positive staining in paraffin-embedded human skin. Anti-Collagen XVII antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human esophagus. Anti-Collagen XVII antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Collagen XVII antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Collagen XVII antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human transitional cell cancer. Anti-Collagen XVII antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in A431 cells (top panel) and negative staining in MCF7 cells (below panel). Anti-Collagen XVII antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Collagen XVII in tumor tissue.
Expression of Collagen XVII in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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