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Rabbit抗原名称
MTCO2分子别名
Cytochrome c oxidase subunit 2; Cytochrome c oxidase polypeptide II; MT-CO2; COII; COX2免疫原
Synthetic PeptideAccession
P00403抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, THP-1, MCF7, K562, Jurkat, COLO 205预测反应种属
(反应种属缩写表)Cz, Or, Gor, Mq, AfGrMk纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:200 Hu
MTCO2 is a subunit of cytochrome c oxidase, which is the last enzyme in the mitochondrial electron transport chain. This enzyme complex drives oxidative phosphorylation and is responsible for the reduction of oxygen to water, a process that is essential for cellular energy metabolism. MTCO2 contributes to cytochrome-c oxidase activity and is predicted to be involved in mitochondrial electron transport from cytochrome c to oxygen, as well as in the positive regulation of vasoconstriction. It is a biomarker of Huntington's disease and stomach cancer, highlighting its importance in human health and disease. MTCO2 also serves as a prognostic marker in certain types of cancer, including liver hepatocellular carcinoma and pancreatic adenocarcinoma.
免疫印迹
WB result of MTCO2 Rabbit pAb
Primary antibody: MTCO2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Lane 6: COLO 205 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 26 kDa
Observed MW: 18 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-MTCO2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-MTCO2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-MTCO2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-MTCO2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of MTCO2 in tumor tissue.
Expression of MTCO2 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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