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Rabbit抗原名称
MTCO2分子别名
Cytochrome c oxidase subunit 2; Cytochrome c oxidase polypeptide II; MT-CO2; COII; COX2免疫原
Synthetic PeptideAccession
P00403抗体类型
Polyclonal antibody抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu阳性样本
HeLa, THP-1, MCF7, K562, Jurkat, COLO 205预测反应种属
(反应种属缩写表)Cz, Or, Gor, Mq, AfGrMk纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
MTCO2 is a subunit of cytochrome c oxidase, which is the last enzyme in the mitochondrial electron transport chain. This enzyme complex drives oxidative phosphorylation and is responsible for the reduction of oxygen to water, a process that is essential for cellular energy metabolism. MTCO2 contributes to cytochrome-c oxidase activity and is predicted to be involved in mitochondrial electron transport from cytochrome c to oxygen, as well as in the positive regulation of vasoconstriction. It is a biomarker of Huntington's disease and stomach cancer, highlighting its importance in human health and disease. MTCO2 also serves as a prognostic marker in certain types of cancer, including liver hepatocellular carcinoma and pancreatic adenocarcinoma.
免疫印迹
WB result of MTCO2 Rabbit pAb
Primary antibody: MTCO2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Lane 6: COLO 205 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 26 kDa
Observed MW: 18 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-MTCO2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-MTCO2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-MTCO2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-MTCO2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of MTCO2 in tumor tissue.
Expression of MTCO2 in human tissue.
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