FMRP Recombinant Rabbit mAb (S-1671-1)

Fragile X messenger ribonucleoprotein 1,Fragile X messenger ribonucleoprotein,Protein FMR-1,FMR1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1156

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

FMRP
分子别名

Fragile X messenger ribonucleoprotein 1; Fragile X messenger ribonucleoprotein; Protein FMR-1; FMR1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

Q06787
克隆号

S-1671-1
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HEK-293, 293T, HeLa, A431, A375, C2C12, NIH/3T3, PC-12
预测反应种属
(反应种属缩写表)

Or
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
IHC-P 1:200 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
IHC-P	1:200	Hu, Ms, Rt

背景介绍

FMRP, or Fragile X Mental Retardation Protein, is a multifunctional RNA-binding protein that plays a crucial role in synaptic development and plasticity in the nervous system. It is widely expressed across all tissues, although at varying levels, and is not restricted to the synapse or neurons. It is involved in regulating the fate of stem cells, being essential for stem cell maintenance and inhibiting stem cell differentiation. FMRP binds to a variety of RNAs, including methylated histone, ribosome, mRNP, and other proteins. It is known to interact with a specific set of mRNAs, aiding in their transport along microtubules and regulating their translation through association with ribosomes. FMRP is established as a translation repressor, but recent studies on mouse and human FXS models suggest that FMRP is involved in regulating almost all aspects of gene expression. It can also enhance the translation of its target mRNAs by binding to the coding sequence and hindering ribosome movement on mRNA. FMRP deficiency is associated with Fragile X Syndrome (FXS), the most common inherited cause of intellectual disability and autism. FMRP has also been implicated in cancer growth and immune evasion.

免疫印迹
- WB result of FMRP Recombinant Rabbit mAb
  Primary antibody: FMRP Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: 293T whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: A431 whole cell lysate 20 µg
  Lane 5: A375 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 71 kDa
  Observed MW: 75 kDa
- WB result of FMRP Recombinant Rabbit mAb
  Primary antibody: FMRP Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C2C12 whole cell lysate 20 µg
  Lane 2: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 71 kDa
  Observed MW: 75 kDa
- WB result of FMRP Recombinant Rabbit mAb
  Primary antibody: FMRP Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 71 kDa
  Observed MW: 75 kDa
免疫沉淀
- FMRP Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating FMRP in 0.4 mg 293T whole cell lysate.
  Western blot was performed on the immunoprecipitate using FMRP Recombinant Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: 293T whole cell lysate 40 µg (Input)
  Lane 2: FMRP Recombinant Rabbit mAb IP in 293T whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in 293T whole cell lysate
  Predicted MW: 71 kDa
  Observed MW: 75 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-FMRP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human testis. Anti-FMRP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-FMRP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse testis. Anti-FMRP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-FMRP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-FMRP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of FMRP in tumor tissue.
- Expression of FMRP in human tissue.
- Expression of FMRP in mouse & rat tissue.