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Rabbit抗原名称
hnRNPK分子别名
Heterogeneous nuclear ribonucleoprotein K; Transformation up-regulated nuclear protein (TUNP); HNRPK免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P61978克隆号
S-1645-45抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, ICC, WB, IP反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, Jurkat, 293T, MDA-MB-231, NIH/3T3, MEF, PC-12预测反应种属
(反应种属缩写表)Bv, Mq, Ck, Rb纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:5000 | Hu, Ms, Rt |
| IP | 1:50 | Hu |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a multifunctional protein that plays a significant role in various cellular processes. It is involved in a wide range of biological processes such as transcription, translation, splicing, chromatin remodeling, and signal transduction. It can interact with numerous nucleic acids and various proteins, influencing cellular functions like proliferation, differentiation, apoptosis, DNA damage repair, and stress and immune responses. HnRNPK has been implicated in the development and progression of various diseases, particularly in cancer. It can act as an oncogene, being overexpressed in cancer tissues compared to non-neoplastic tissues, and its expression level is related to the prognosis of different types of malignancies. However, it has also been identified as a tumor suppressor, important for the activation of the p53/p21 pathway. HnRNPK may play a role in regulating 3'-end RNA processing and transcription termination, particularly through its interaction with the 5'-3' exonuclease XRN2. HnRNPK has been found to be related to the regulation of long noncoding RNAs (lncRNAs) such as Neat1, Lncenc1, and Xist, which are involved in various cellular events, including cancer development. Interestingly, hnRNPK has also been associated with skeletal abnormalities and is essential for the formation of osteoclasts, highlighting its importance in the skeletal system.
免疫印迹
WB result of hnRNPK Recombinant Rabbit mAb
Primary antibody: hnRNPK Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: MDA-MB-231 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 51 kDa
Observed MW: 50~65 kDaWB result of hnRNPK Recombinant Rabbit mAb
Primary antibody: hnRNPK Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: MEF whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 51 kDa
Observed MW: 60 kDaWB result of hnRNPK Recombinant Rabbit mAb
Primary antibody: hnRNPK Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 51 kDa
Observed MW: 60 kDa
免疫沉淀
hnRNPK Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating hnRNPK in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using hnRNPK Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 µg (Input)
Lane 2: hnRNPK Recombinant Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 51 kDa
Observed MW: 60 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-hnRNPK antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-hnRNPK antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-hnRNPK antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-hnRNPK antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-hnRNPK antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- hnRNPK antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of hnRNPK in tumor tissue.
Expression of hnRNPK in human tissue.
Expression of hnRNPK in mouse & rat tissue.
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