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Rabbit抗原名称
SHMT2/SHMT分子别名
Serine hydroxymethyltransferase, mitochondrial; Glycine hydroxymethyltransferase; Serine methylase免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
P34897抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HepG2, Jurkat, MDA-MB-231, HeLa, mouse kidney, rat kidney纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:200 Hu, Ms, Rt
SHMT2 is a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. It is primarily responsible for glycine synthesis and is considered the main source of intracellular glycine. SHMT2 is mainly localized in mitochondria and is involved in the conversion of serine to glycine, which is crucial for one-carbon unit metabolism. This process supports the synthesis of thymidine and purines, promoting tumor growth. SHMT2 is highly expressed in various human tumors, including hepatocellular carcinoma, breast cancer, and non-small-cell lung cancer (NSCLC). Its elevated expression is associated with poor prognosis, and it may serve as a prognostic marker and a target for anticancer therapies. In cancer cells, SHMT2 supports tumor cell proliferation by maintaining redox balance and cell survival. It is involved in metabolic reprogramming, which is a significant hallmark of cancer. Genetic variations in the SHMT2 gene are significantly associated with changes in metabolic parameters such as circulating lipids, body mass indexes, and diabetic indicators, suggesting that SHMT2 may influence the development and outcome of metabolic syndrome in humans.
免疫印迹
WB result of SHMT2/SHMT Rabbit pAb
Primary antibody: SHMT2/SHMT Rabbit pAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: MDA-MB-231 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 56 kDa
Observed MW: 54 kDaWB result of SHMT2/SHMT Rabbit pAb
Primary antibody: SHMT2/SHMT Rabbit pAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 56 kDa
Observed MW: 54 kDaWB result of SHMT2/SHMT Rabbit pAb
Primary antibody: SHMT2/SHMT Rabbit pAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 56 kDa
Observed MW: 54 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-SHMT2/SHMT antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-SHMT2/SHMT antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SHMT2/SHMT antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of SHMT2/SHMT in tumor tissue.
Expression of SHMT2/SHMT in human tissue.
Expression of SHMT2/SHMT in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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