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Rabbit抗原名称
PLCG1分子别名
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II (PLC-II); Phospholipase C-gamma-1 (PLC-gamma-1); PLC1免疫原
Synthetic Peptide细胞定位
Cell projectionAccession
P19174克隆号
S-978-37抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HepG2, Jurkat, NIH/3T3, mouse brain预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:4000 Hu, Ms IP 1:50 Hu IHC-P 1:100 Hu, Ms, Rt ICC 1:2000 Hu ICFCM 1:50 Hu
PLCG1, or Phospholipase C gamma 1, is a member of the phospholipase C (PLC) family and plays a critical role in intracellular signal transduction. PLCG1 generates two second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), by hydrolyzing the membrane phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2). This process regulates various cellular and physiological functions, including cell proliferation, survival, migration, invasion, and angiogenesis, which are essential for tumorigenesis. The role of PLCG1 in various human cancers has been extensively studied, including breast, lung, pancreatic, gastric, prostate, and ovarian cancers. It intersects with multiple oncogenic drivers and signaling pathways, such as PI3K, AKT, HIF1-α, and the RAF/MEK/ERK cascade. Furthermore, overexpression of PLCG1 is associated with tumor growth and poor prognosis in adult IDH wild-type low-grade glioma (LGG) patients. In terms of immune modulation, PLCG1 is also a crucial enzyme that regulates the NF-κB, extracellular signal-regulated kinase, mitogen-activated protein kinase, and nuclear factor of activated T-cells signaling pathways.
免疫印迹
WB result of PLCG1 Recombinant Rabbit mAb
Primary antibody: PLCG1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 149 kDa
Observed MW: 150 kDaWB result of PLCG1 Recombinant Rabbit mAb
Primary antibody: PLCG1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 149 kDa
Observed MW: 150 kDa
流式分析
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) labelling PLCG1 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
PLCG1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating PLCG1 in 0.4 mg Jurkat whole cell lysate.
Western blot was performed on the immunoprecipitate using PLCG1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate 20 µg (Input)
Lane 2: PLCG1 Rabbit mAb IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
Predicted MW: 149 kDa
Observed MW: 150 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PLCG1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-PLCG1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-PLCG1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-PLCG1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-PLCG1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-PLCG1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Jurkat cells. Anti-PLCG1 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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