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Rabbit抗原名称
Vitamin D Receptor分子别名
Vitamin D3 receptor; VDR; 1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1; NR1I1免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P11473克隆号
S-1719-7抗体类型
Recombinant mAb抗体同种型
IgG应用
ICC, WB, IP反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, T-47D, HL-60, DU 145, THP-1, HT-29, LOVO, NIH/3T3, mouse kidney, rat kidney预测反应种属
(反应种属缩写表)Bv, Pg, CtTa, Ck纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Hu |
| ICC | 1:500 | Hu |
VDR is a nuclear transcription factor encoded by the VDR gene, which is a member of the steroid-thyroid-vitamin D receptor gene superfamily of nuclear transcription factors. It has multiple domains, including an amino-terminal segment (domains A and B), a DNA binding domain (C) with two zinc fingers, a hinge region (D), and a carboxyl terminal domain (E) with ligand and retinoid X receptor α (RXRα) binding sites. VDR acts as a ligand-activated transcription factor that binds to the vitamin D response element (VDRE) in the promoter regions of target genes, regulating their transcription. VDR is widely expressed in various human tissues, including the intestine, kidney, bone, skin, and immune cells. It has been reported to have more than 1000 target genes and is found in most human tissues. VDR is involved in calcium and phosphorus homeostasis, bone health, immune regulation, and has been implicated in the pathology of at least 17 types of cancer, autoimmune diseases, diabetes, osteoarthritis, and periodontal disease. It also plays a role in the central nervous system, with VDR gene polymorphisms and vitamin D deficiency being associated with an increased susceptibility to Alzheimer's disease and Parkinson's disease.
免疫印迹
WB result of Vitamin D Receptor Recombinant Rabbit mAb
Primary antibody: Vitamin D Receptor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: T-47D whole cell lysate 20 µg
Lane 3: HL-60 whole cell lysate 20 µg
Lane 4: DU 145 whole cell lysate 20 µg
Lane 5: THP-1 whole cell lysate 20 µg
Lane 6: HT-29 whole cell lysate 20 µg
Lane 7: LOVO whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDaWB result of Vitamin D Receptor Recombinant Rabbit mAb
Primary antibody: Vitamin D Receptor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDaWB result of Vitamin D Receptor Recombinant Rabbit mAb
Primary antibody: Vitamin D Receptor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa
免疫沉淀
Vitamin D Receptor Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Vitamin D Receptor in 0.4 mg T-47D whole cell lysate.
Western blot was performed on the immunoprecipitate using Vitamin D Receptor Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: T-47D whole cell lysate 40 µg (Input)
Lane 2: Vitamin D Receptor Recombinant Rabbit mAb IP in T-47D whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in T-47D whole cell lysate
Predicted MW: 48 kDa
Observed MW: 48 kDa
免疫细胞化学
ICC shows positive staining in A431 cells. Anti-Vitamin D Receptor antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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