Vitamin D Receptor Recombinant Rabbit mAb (S-1719-7)

Vitamin D3 receptor,VDR,1,25-dihydroxyvitamin D3 receptor,Nuclear receptor subfamily 1 group I member 1,NR1I1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1146

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Vitamin D Receptor
分子别名

Vitamin D3 receptor; VDR; 1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1; NR1I1
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

P11473
克隆号

S-1719-7
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, T-47D, HL-60, DU 145, THP-1, HT-29, LOVO, NIH/3T3, mouse kidney, rat kidney
预测反应种属
(反应种属缩写表)

Bv, Pg, CtTa, Ck
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu
ICC	1:500	Hu

背景介绍

VDR is a nuclear transcription factor encoded by the VDR gene, which is a member of the steroid-thyroid-vitamin D receptor gene superfamily of nuclear transcription factors. It has multiple domains, including an amino-terminal segment (domains A and B), a DNA binding domain (C) with two zinc fingers, a hinge region (D), and a carboxyl terminal domain (E) with ligand and retinoid X receptor α (RXRα) binding sites. VDR acts as a ligand-activated transcription factor that binds to the vitamin D response element (VDRE) in the promoter regions of target genes, regulating their transcription. VDR is widely expressed in various human tissues, including the intestine, kidney, bone, skin, and immune cells. It has been reported to have more than 1000 target genes and is found in most human tissues. VDR is involved in calcium and phosphorus homeostasis, bone health, immune regulation, and has been implicated in the pathology of at least 17 types of cancer, autoimmune diseases, diabetes, osteoarthritis, and periodontal disease. It also plays a role in the central nervous system, with VDR gene polymorphisms and vitamin D deficiency being associated with an increased susceptibility to Alzheimer's disease and Parkinson's disease.

免疫印迹
- WB result of Vitamin D Receptor Recombinant Rabbit mAb
  Primary antibody: Vitamin D Receptor Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: T-47D whole cell lysate 20 µg
  Lane 3: HL-60 whole cell lysate 20 µg
  Lane 4: DU 145 whole cell lysate 20 µg
  Lane 5: THP-1 whole cell lysate 20 µg
  Lane 6: HT-29 whole cell lysate 20 µg
  Lane 7: LOVO whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 48 kDa
- WB result of Vitamin D Receptor Recombinant Rabbit mAb
  Primary antibody: Vitamin D Receptor Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse kidney lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 48 kDa
- WB result of Vitamin D Receptor Recombinant Rabbit mAb
  Primary antibody: Vitamin D Receptor Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat kidney lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 48 kDa
免疫沉淀
- Vitamin D Receptor Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Vitamin D Receptor in 0.4 mg T-47D whole cell lysate.
  Western blot was performed on the immunoprecipitate using Vitamin D Receptor Recombinant Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: T-47D whole cell lysate 40 µg (Input)
  Lane 2: Vitamin D Receptor Recombinant Rabbit mAb IP in T-47D whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in T-47D whole cell lysate
  Predicted MW: 48 kDa
  Observed MW: 48 kDa
免疫细胞化学
- ICC shows positive staining in A431 cells. Anti-Vitamin D Receptor antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).