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RPA32/RPA2 Rabbit Polyclonal Antibody

Replication protein A 32 kDa subunit,RP-A p32,Replication factor A protein 2 (RF-A protein 2),Replication protein A 34 kDa subunit (RP-A p34),REPA2,RPA34

Datasheet COA

价格￥400.00 （供应商现货： 3-5个工作日）

货号 S0B1143

规格

25μl100μl1ml

数量

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

RPA32
分子别名

Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2 (RF-A protein 2); Replication protein A 34 kDa subunit (RP-A p34); REPA2; RPA34
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P15927
抗体类型

Polyclonal antibody
抗体同种型

IgG
应用

WB ,
IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50:00
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, Jurkat, HUVEC, NIH/3T3, C6
预测反应种属
(反应种属缩写表)

Or
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50:00

背景介绍

RPA32, also known as the 32 kDa subunit of Replication Protein A (RPA), is an essential component of the RPA complex. RPA is a highly conserved single-stranded DNA (ssDNA) binding protein in eukaryotes, composed of three subunits: RPA70, RPA32, and RPA14. RPA32 binds to ssDNA during DNA replication and repair, and plays a crucial role in DNA damage response. Particularly, the phosphorylation of threonine 21 (Thr21) in RPA32 is vital for effective DNA repair, especially in homologous recombination (HR), as it enhances RPA's binding affinity to ssDNA and promotes the recruitment of Rad51, a key player in HR that contributes to genomic stability.

免疫印迹
- WB result of RPA32/RPA2 Rabbit pAb
  Primary antibody: RPA32/RPA2 Rabbit pAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: HUVEC whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa
  Observed MW: 29 kDa
- WB result of RPA32/RPA2 Rabbit pAb
  Primary antibody: RPA32/RPA2 Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa
  Observed MW: 29 kDa
- WB result of RPA32/RPA2 Rabbit pAb
  Primary antibody: RPA32/RPA2 Rabbit pAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 29 kDa
  Observed MW: 29 kDa
免疫沉淀
- RPA32/RPA2 Rabbit Polyclonal Antibody at 1/50 dilution (1 µg) immunoprecipitating RPA32/RPA2 in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using RPA32/RPA2 Rabbit Polyclonal Antibody at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: RPA32/RPA2 Rabbit Polyclonal Antibody IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 29 kDa
  Observed MW: 29 kDa