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VEGF Receptor 2/FLK-1 Recombinant Rabbit mAb (S-1519-34)

Vascular endothelial growth factor receptor 2,VEGFR-2,Fetal liver kinase 1 (FLK-1),Kinase insert domain receptor (KDR),Protein-tyrosine kinase receptor flk-1,CD309,KDR

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1138

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

VEGF Receptor/FLK-1
分子别名

Vascular endothelial growth factor receptor 2; VEGFR-2; Fetal liver kinase 1 (FLK-1); Kinase insert domain receptor (KDR); Protein-tyrosine kinase receptor flk-1; CD309; KDR
免疫原

Recombinant Protein
细胞定位

Cell membrane, Cell junction
Accession

P35968
克隆号

S-1519-34
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

HUVEC
预测反应种属
(反应种属缩写表)

P. paniscus, P. troglodytes
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IP 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
IP	1:50	Hu

背景介绍

VEGF Receptor 2 (VEGFR2), also known as kinase insert domain receptor (KDR) in humans, is a crucial tyrosine kinase receptor involved in angiogenesis, the formation of new blood vessels. It plays a significant role in various physiological and pathological processes, including development, wound healing, and tumor growth. VEGFR2 is activated upon binding to VEGF (Vascular Endothelial Growth Factor), leading to a series of downstream signaling events that promote endothelial cell proliferation, migration, and survival. This receptor is composed of an extracellular ligand-binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. Upon VEGF binding, VEGFR2 undergoes dimerization and autophosphorylation, which activates various signaling pathways such as PI3K/Akt, MAPK/ERK, and others.

免疫印迹
- WB result of VEGF Receptor 2/FLK-1 Recombinant Rabbit mAb
  Primary antibody: VEGF Receptor 2/FLK-1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HUVEC whole cell lysate 20 µg
  Negative control: HeLa whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 152 kDa
  Observed MW: 200~260 kDa
免疫沉淀
- VEGF Receptor 2/FLK-1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating VEGF Receptor 2/FLK-1 in 0.4 mg HUVEC whole cell lysate.
  Western blot was performed on the immunoprecipitate using VEGF Receptor 2/FLK-1 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HUVEC whole cell lysate 20 µg (Input)
  Lane 2: VEGF Receptor 2/FLK-1 Rabbit mAb IP in HUVEC whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HUVEC whole cell lysate
  Predicted MW: 152 kDa
  Observed MW: 200~260 kDa
  This blot was developed with high sensitivity substrate