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Rabbit抗原名称
TAF15分子别名
TATA-binding protein-associated factor 2N; 68 kDa TATA-binding protein-associated factor (TAF(II)68; TAFII68), RNA-binding protein 56; RBP56; TAF2N免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q92804克隆号
S-1508-53抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, 293T, Caco-2, NIH/3T3, C6纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IP 1:50 Hu IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu, Ms
TAF15, or TATA-box binding protein associated factor 15, is an RNA-binding protein belonging to the TET family. It plays a significant role in RNA polymerase II gene transcription as a component of the multi-subunit transcription initiation factor TFIID complexes. TAF15 is involved in various cellular processes, including RNA splicing, transcription, mRNA transport, signaling, modification, translation, and the maintenance of genome integrity. This protein is mainly present in the cell nucleus but is also found in the cell surface and cytoplasm, indicating its extended functionality in regulation and interaction with various proteins. TAF15 has been implicated in the progression of several types of cancer, including extraskeletal myxoid sarcomas, leukemia, neuroblastoma, and lung squamous cell carcinoma. It can influence a large number of genes related to the cell cycle and cell death, and its overexpression has been associated with worsened survival in non-small cell lung cancer patients. Furthermore, TAF15 is involved in drug tolerance of several cancer cells and can activate the RAF1/MEK/ERK signaling pathway, contributing to cell proliferation, migration, and invasion in gastric cancer. These functions make TAF15 a potential molecular diagnostic marker or therapeutic target for various cancers.
免疫印迹
WB result of TAF15 Recombinant Rabbit mAb
Primary antibody: TAF15 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 70 kDaWB result of TAF15 Recombinant Rabbit mAb
Primary antibody: TAF15 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 70 kDaWB result of TAF15 Recombinant Rabbit mAb
Primary antibody: TAF15 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 62 kDa
免疫沉淀
TAF15 Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating TAF15 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using TAF15 Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 µg (Input)
Lane 2: TAF15 Recombinant Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 62 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-TAF15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-TAF15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-TAF15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-TAF15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-TAF15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-TAF15 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- TAF15 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- TAF15 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of TAF15 in tumor tissue.
Expression of TAF15 in human tissue.
Expression of TAF15 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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