Rat Anti-Mouse F4/80 Antibody (S-R537)
AGRE1,Adhesion G protein-coupled receptor E1,Cell surface glycoprotein F4/80,EGF-like module receptor 1,EGF-like module-containing mucin-like hormone receptor-like 1,EMR1 hormone receptor,Adgre1,Emr1,Gpf480,F4/80
货号： S0B1135
- 价格： ￥600
- 规格：
- 数量：
大包装询价 产品介绍 评论(0)
宿主来源
Rat抗原名称
F4/80分子别名
Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; Gpf480; Adgre1细胞定位
Cell membraneAccession
Q61549克隆号
S-R537抗体类型
Rat mAb抗体同种型
IgG2a,k应用
FCM, ICC反应种属 ?
Ms阳性样本
RAW 264.7纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| ICC | 1:500 | Ms |
| FCM | 1:2000 | Ms |
F4/80, also known as EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1), is a cell surface glycoprotein that is a member of the EGF-TM7 protein family. It is primarily used as a marker for mature macrophages in mice and shows significant expression changes during the maturation and activation of these cells. F4/80 is expressed on various mature macrophage populations, including Kupffer cells, Langerhans cells, microglia, and macrophages located in the peritoneum, intestinal lamina propria, splenic red pulp, lung, thymus, and bone marrow stroma. This antigen is encoded by the Adgre1 gene, which is a seven transmembrane G protein-coupled receptor with an extracellular domain containing repeated Epidermal Growth Factor (EGF)-like calcium binding domains. The F4/80 antigen is not only crucial for the phenotypic characterization of macrophages but also plays a role in immune regulation, as indicated by studies showing its necessity for inducing CD8+ regulatory T cells responsible for peripheral immune tolerance.
流式分析
Flow cytometric analysis of C2C12 (Mouse myoblasts myoblast, left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, right) labelling Mouse F4/80 antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 647 was used as the secondary antibody.
Negative control: C2C12
Flow cytometric analysis of mouse peritoneal exudates cells labelling Mouse F4/80 antibody at 1/2000 (0.1 μg) dilution/ (Right panel) compared with a Rat IgG2a, κ Isotype Control / (Left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD11b - Brilliant Violet 421™ Antibody separately.
免疫细胞化学
ICC shows positive staining in RAW 264.7 cells (top panel) and negative staining in C2C12 cells (below panel). Rat Anti-Mouse F4/80 Antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
评论(0)