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Rat Anti-Mouse F4/80 Antibody (S-R537)

Adhesion G protein-coupled receptor E1,Cell surface glycoprotein F4/80,EGF-like module receptor 1,EGF-like module-containing mucin-like hormone receptor-like 1,EMR1 hormone receptor,Emr1,Gpf480,Adgre1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1135

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rat
抗原名称

F4/80
分子别名

Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; Gpf480; Adgre1
细胞定位

Cell membrane
Accession

Q61549
克隆号

S-R537
抗体类型

Rat mAb
抗体同种型

IgG2a,k
反应种属 ?

Ms
阳性样本

RAW 264.7
纯化方式

Protein G
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS pH7.4
储存条件

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用

FCM

ICC ?
稀释度

应用稀释度推荐种属

ICC 1:500 Ms
FCM 1:2000 Ms

应用	稀释度	推荐种属
ICC	1:500	Ms
FCM	1:2000	Ms

背景介绍

F4/80, also known as EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1), is a cell surface glycoprotein that is a member of the EGF-TM7 protein family. It is primarily used as a marker for mature macrophages in mice and shows significant expression changes during the maturation and activation of these cells. F4/80 is expressed on various mature macrophage populations, including Kupffer cells, Langerhans cells, microglia, and macrophages located in the peritoneum, intestinal lamina propria, splenic red pulp, lung, thymus, and bone marrow stroma. This antigen is encoded by the Adgre1 gene, which is a seven transmembrane G protein-coupled receptor with an extracellular domain containing repeated Epidermal Growth Factor (EGF)-like calcium binding domains. The F4/80 antigen is not only crucial for the phenotypic characterization of macrophages but also plays a role in immune regulation, as indicated by studies showing its necessity for inducing CD8+ regulatory T cells responsible for peripheral immune tolerance.

流式分析
- Flow cytometric analysis of C2C12 (Mouse myoblasts myoblast, left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, right) labelling Mouse F4/80 antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 647 was used as the secondary antibody.
  Negative control: C2C12
- Flow cytometric analysis of mouse peritoneal exudates cells labelling Mouse F4/80 antibody at 1/2000 (0.1 μg) dilution/ (Right panel) compared with a Rat IgG2a, κ Isotype Control / (Left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD11b - Brilliant Violet 421™ Antibody separately.
免疫细胞化学
- ICC shows positive staining in RAW 264.7 cells (top panel) and negative staining in C2C12 cells (below panel). Rat Anti-Mouse F4/80 Antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).